ArticleJournal of bacteriology2002
Mutations in the CCGTTCACA DnaA box of Mycobacterium tuberculosis oriC that abolish replication of oriC plasmids are tolerated on the chromosome.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Depletion of tRNA CCA-adding enzyme in Mycobacterium tuberculosis leads to polyadenylation of transcripts and precursor tRNAs.Scientific reports · 2023Article
- Novel Isoniazid-Carborane Hybrids Active in Vitro AgainstPharmaceuticals (Basel, Switzerland) · 2020Article
- MtrA Response Regulator Controls Cell Division and Cell Wall Metabolism and Affects Susceptibility of Mycobacteria to the First Line Antituberculosis Drugs.Frontiers in microbiology · 2018Article
- Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex.Molecular biology reports · 2017Review
- PdtaS Deficiency Affects Resistance of Mycobacteria to Ribosome Targeting Antibiotics.Frontiers in microbiology · 2017Article
- In Vivo IS6110 Profile Changes in a Mycobacterium tuberculosis Strain as Determined by Tracking over 14 Years.Journal of clinical microbiology · 2015Article
- The deletion of rnhB in Mycobacterium smegmatis does not affect the level of RNase HII substrates or influence genome stability.PloS one · 2015Article
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- Localization of acidic phospholipid cardiolipin and DnaA in mycobacteria.Tuberculosis (Edinburgh, Scotland) · 2011Article
- Mycobacterium tuberculosis origin of replication and the promoter for immunodominant secreted antigen 85B are the targets of MtrA, the essential response regulator.The Journal of biological chemistry · 2010Article
- The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication.Molecular microbiology · 2009Article
- Synchronous replication initiation in novel Mycobacterium tuberculosis dnaA cold-sensitive mutants.Molecular microbiology · 2009Article
- Mycobacterium tuberculosis DnaA initiator protein: purification and DNA-binding requirements.The Biochemical journal · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The origin of replication (oriC) region in some clinical strains of Mycobacterium tuberculosis is a hot spot for IS6110 elements. To understand how clinical strains with insertions in oriC can replicate their DNA, we characterized the oriC regions of some clinical strains. Using a plasmid-based oriC-dependent replication assay, we showed that IS6110 insertions that disrupted the DnaA box sequence CCGTTCACA abolished oriC activity in M. tuberculosis. Furthermore, by using a surface plasmon resonance technique we showed that purified M. tuberculosis DnaA protein binds native but not mutant DnaA box sequence, suggesting that stable interactions of the DnaA protein with the CCGTTCACA DnaA box are crucial for replication of oriC plasmids in vivo. Replacement by homologous recombination of the CCGTTCACA DnaA box sequence of the laboratory strain M. tuberculosis H37Ra with a mutant sequence did not result in nonviability. Together, these results suggest that M. tuberculosis strains have evolved mechanisms to tolerate mutations in the oriC region and that functional requirements for M. tuberculosis oriC replication are different for chromosomes and plasmids.
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