ArticleJournal of bacteriology2002
Analysis of DNA regulatory elements required for expression of the Legionella pneumophila icm and dot virulence genes.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Evaluating EcxR for Its Possible Role inInternational journal of molecular sciences · 2022Article
- Legionella pneumophila OxyR Is a Redundant Transcriptional Regulator That Contributes to Expression Control of the Two-Component CpxRA System.Journal of bacteriology · 2017Article
- ClpP-deletion impairs the virulence of Legionella pneumophila and the optimal translocation of effector proteins.BMC microbiology · 2016Article
- Alveolar macrophages and neutrophils are the primary reservoirs for Legionella pneumophila and mediate cytosolic surveillance of type IV secretion.Infection and immunity · 2014Article
- Searching algorithm for type IV secretion system effectors 1.0: a tool for predicting type IV effectors and exploring their genomic context.Nucleic acids research · 2013Article
- Cell biology of infection by Legionella pneumophila.Microbes and infection · 2013Review
- Evidence of the presence of a functional Dot/Icm type IV-B secretion system in the fish bacterial pathogen Piscirickettsia salmonis.PloS one · 2013Article
- Virulence phenotypes of Legionella pneumophila associated with noncoding RNA lpr0035.Infection and immunity · 2012Article
- Coxiella burnetii type IVB secretion system region I genes are expressed early during the infection of host cells.FEMS microbiology letters · 2010Article
- bdhA-patD operon as a virulence determinant, revealed by a novel large-scale approach for identification of Legionella pneumophila mutants defective for amoeba infection.Applied and environmental microbiology · 2009Article
- The PmrA/PmrB two-component system of Legionella pneumophila is a global regulator required for intracellular replication within macrophages and protozoa.Infection and immunity · 2009Article
- Identification of a hypervariable region containing new Legionella pneumophila Icm/Dot translocated substrates by using the conserved icmQ regulatory signature.Infection and immunity · 2008Article
- An in vivo gene deletion system for determining temporal requirement of bacterial virulence factors.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Lgt: a family of cytotoxic glucosyltransferases produced by Legionella pneumophila.Journal of bacteriology · 2008Article
- Regulation of type IV secretion apparatus genes during Ehrlichia chaffeensis intracellular development by a previously unidentified protein.Journal of bacteriology · 2008Article
- A pair of highly conserved two-component systems participates in the regulation of the hypervariable FIR proteins in different Legionella species.Journal of bacteriology · 2007Article
- Additive effect on intracellular growth by Legionella pneumophila Icm/Dot proteins containing a lipobox motif.Infection and immunity · 2005Article
- A specific genomic location within the icm/dot pathogenesis region of different Legionella species encodes functionally similar but nonhomologous virulence proteins.Infection and immunity · 2004Article
- Differentiate to thrive: lessons from the Legionella pneumophila life cycle.Molecular microbiology · 2004Review
- Characterization of the icmH and icmF genes required for Legionella pneumophila intracellular growth, genes that are present in many bacteria associated with eukaryotic cells.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the regulation of the Legionella pneumophila icm and dot genes required for intracellular growth, a series of nine icm::lacZ fusions were constructed. These icm::lacZ fusions were found to have different levels of expression in L. pneumophila, and five of them were more highly expressed at stationary phase than at exponential phase. When the expression of these fusions in Escherichia coli was tested, all of them were found to be expressed but three of them had dramatic changes in their levels of expression in comparison to those in L. pneumophila. Site-directed and PCR random mutagenesis with these icm::lacZ fusions was used to identify DNA regulatory elements of icm genes. Four icm genes (icmT, icmP, icmQ, and icmM) that had low levels of expression in L. pneumophila were found to contain a 6-bp sequence (TATACT) essential for their expression. This sequence was shown by primer extension to serve as their -10 promoter elements. A similar sequence, which constitutes the -10 promoter elements of the icmV, icmW, and icmR genes which had high levels of expression in L. pneumophila, was also identified. In addition, regulatory elements that probably serve as binding sites for transcription regulators were found in these genes. Altogether, 12 regulatory elements, 7 of which constitute the -10 promoter elements of the icm genes, were found. Even though all the icm and dot genes are part of one system required for L. pneumophila intracellular growth and even though their promoters are probably recognized by the vegetative sigma factor, it seems that they are subjected to different regulation mediated by several regulatory factors.
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