ArticleBiophysical journal2002
Anisotropic diffusion in mitral cell dendrites revealed by fluorescence correlation spectroscopy.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 51 citations in OpenAlex.
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- Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes.The Journal of biological chemistry · 2020Article
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- Quantifying translational mobility in neurons: comparison between current optical techniques.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Review
- Microscale diffusion properties of the cartilage pericellular matrix measured using 3D scanning microphotolysis.Journal of biomechanical engineering · 2008Article
- Anisotropic diffusion of fluorescently labeled ATP in rat cardiomyocytes determined by raster image correlation spectroscopy.American journal of physiology. Cell physiology · 2008Article
- Diffusional anisotropy in collagenous tissues: fluorescence imaging of continuous point photobleaching.Biophysical journal · 2006Article
- Fluorescence correlation spectroscopy simulations of photophysical phenomena and molecular interactions: a molecular dynamics/monte carlo approach.The journal of physical chemistry. B · 2006Article
- Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization.Biophysical journal · 2005Article
- Extracellular space diffusion in central nervous system: anisotropic diffusion measured by elliptical surface photobleaching.Biophysical journal · 2005Article
- Sizing-up finite fluorescent particles with nanometer-scale precision by convolution and correlation image analysis.European biophysics journal : EBJ · 2005Article
- Action potential backpropagation and multiglomerular signaling in the rat vomeronasal system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- High- and low-mobility populations of HP1 in heterochromatin of mammalian cells.Molecular biology of the cell · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Fluorescence correlation spectroscopy (FCS) can be used to measure kinetic properties of single molecules in drops of solution or in cells. Here we report on FCS measurements of tetramethylrhodamine (TMR)-dextran (10 kDa) in dendrites of cultured mitral cells of Xenopus laevis tadpoles. To interpret such measurements correctly, the plasma membrane as a boundary of diffusion has to be taken into account. We show that the fluorescence data recorded from dendrites are best described by a model of anisotropic diffusion. As compared to diffusion in water, diffusion of the 10-kDa TMR-dextran along the dendrite is slowed down by a factor 1.1-2.1, whereas diffusion in lateral direction is 10-100 times slower. The dense intradendritic network of microtubules oriented parallel to the dendrite is discussed as a possible basis for the observed anisotropy. In somata, diffusion was found to be isotropic in three dimensions and 1.2-2.6 times slower than in water.
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