ArticleBiophysical journal2002
Thermodynamically irreversible gating of ryanodine receptors in situ revealed by stereotyped duration of release in Ca(2+) sparks.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The architecture and function of cardiac dyads.Biophysical reviews · 2020Review
- Calcium-induced release of calcium in muscle: 50 years of work and the emerging consensus.The Journal of general physiology · 2018Review
- Ryanodine receptor cluster fragmentation and redistribution in persistent atrial fibrillation enhance calcium release.Cardiovascular research · 2015Article
- Article
- Calcium antagonists in myocardial ischemia/reperfusion--update 2012.Wiener medizinische Wochenschrift (1946) · 2012Review
- Properties of Ca2+ sparks revealed by four-dimensional confocal imaging of cardiac muscle.The Journal of general physiology · 2012Article
- Quarky calcium release in the heart.Circulation research · 2011Article
- Minding the calcium store: Ryanodine receptor activation as a convergent mechanism of PCB toxicity.Pharmacology & therapeutics · 2010Review
- Dynamic interreceptor coupling contributes to the consistent open duration of ryanodine receptors.Biophysical journal · 2009Article
- Calcium-dependent inactivation terminates calcium release in skeletal muscle of amphibians.The Journal of general physiology · 2008Article
- Concerted vs. sequential. Two activation patterns of vast arrays of intracellular Ca2+ channels in muscle.The Journal of general physiology · 2005Article
- Temperature dependence and thermodynamic properties of Ca2+ sparks in rat cardiomyocytes.Biophysical journal · 2005Article
- Calcium regulation of single ryanodine receptor channel gating analyzed using HMM/MCMC statistical methods.The Journal of general physiology · 2004Article
- Diastolic calcium release controls the beating rate of rabbit sinoatrial node cells: numerical modeling of the coupling process.Biophysical journal · 2004Article
- The quantal nature of Ca2+ sparks and in situ operation of the ryanodine receptor array in cardiac cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A mathematical analysis of the generation and termination of calcium sparks.Biophysical journal · 2004Article
- Imaging single cardiac ryanodine receptor Ca2+ fluxes in lipid bilayers.Biophysical journal · 2004Article
- Polymorphism of Ca2+ sparks evoked from in-focus Ca2+ release units in cardiac myocytes.Biophysical journal · 2004Article
- Intracellular Ca(2+) release as irreversible Markov process.Biophysical journal · 2002Article
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8 authors.
Funding
Abstract
For a single or a group of Markov channels gating reversibly, distributions of open and closed times should be the sum of positively weighted decaying exponentials. Violation of this microscopic reversibility has been demonstrated previously on a number of occasions at the single channel level, and has been attributed to possible channel coupling to external sources of free energy. Here we show that distribution of durations of Ca(2+) release underlying Ca(2+) sparks in intact cardiac myocytes exhibits a prominent mode at approximately 8 ms. Analysis of the cycle time for repetitive sparks at hyperactive sites revealed no intervals briefer than approximately 35 ms and a mode at approximately 90 ms. These results indicate that, regardless of whether Ca(2+) sparks are single-channel or multi-channel in origin, they are generated by thermodynamically irreversible stochastic processes. In contrast, data from planar lipid bilayer experiments were consistent with reversible gating of RyR under asymmetric cis (4 microM) and trans Ca(2+) (10 mM), suggesting that the irreversibility for Ca(2+) spark genesis may reside at a supramolecular level. Modeling suggests that Ca(2+)-induced Ca(2+) release among adjacent RyRs may couple the external energy derived from Ca(2+) gradients across the SR to RyR gating in situ, and drive the irreversible generation of Ca(2+) sparks.
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