ArticleMolecular and cellular biology2002
Drosophila Mi-2 negatively regulates dDREF by inhibiting its DNA-binding activity.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Sexually dimorphic mechanisms of VGLUT-mediated protection from dopaminergic neurodegeneration.bioRxiv : the preprint server for biology · 2025Article
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Vesicular glutamate transporter modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration.Aging cell · 2021Article
- Epigenetic regulation of drosophila germline stem cell maintenance and differentiation.Developmental biology · 2021Review
- ZBED1/DREF: A transcription factor that regulates cell proliferation.Oncology letters · 2020Review
- DREF Genetically Counteracts Mi-2 and Caf1 to Regulate Adult Stem Cell Maintenance.PLoS genetics · 2019Article
- ATP-Dependent Chromatin Remodeling During Cortical Neurogenesis.Frontiers in neuroscience · 2018Review
- Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α.The Journal of biological chemistry · 2016Article
- The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis.The Plant cell · 2014Article
- Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.Oncogenesis · 2014Article
- Arabidopsis chromatin remodeling factor PICKLE interacts with transcription factor HY5 to regulate hypocotyl cell elongation.The Plant cell · 2013Article
- The chromatin remodeling complex NuRD establishes the poised state of rRNA genes characterized by bivalent histone modifications and altered nucleosome positions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway.American journal of cancer research · 2012Article
- Genetic screening for modifiers of the DREF pathway in Drosophila melanogaster: identification and characterization of HP6 as a novel target of DREF.Nucleic acids research · 2009Article
- Transcriptional regulation of the Drosophila moira and osa genes by the DREF pathway.Nucleic acids research · 2008Article
- hDREF regulates cell proliferation and expression of ribosomal protein genes.Molecular and cellular biology · 2007Article
- ATP-dependent chromatin remodeling complexes in Drosophila.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2006Review
- DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster DNA replication-related element (DRE) factor (dDREF) is a transcriptional regulatory factor required for the expression of genes carrying the 5'-TATCGATA DRE. dDREF has been reported to bind to a sequence in the chromatin boundary element, and thus, dDREF may play a part in regulating insulator activity. To generate further insights into dDREF function, we carried out a Saccharomyces cerevisiae two-hybrid screening with DREF polypeptide as bait and identified Mi-2 as a DREF-interacting protein. Biochemical analyses revealed that the C-terminal region of Drosophila Mi-2 (dMi-2) specifically binds to the DNA-binding domain of dDREF. Electrophoretic mobility shift assays showed that dMi-2 thereby inhibits the DNA-binding activity of dDREF. Ectopic expression of dDREF and dMi-2 in eye imaginal discs resulted in severe and mild rough-eye phenotypes, respectively, whereas flies simultaneously expressing both proteins exhibited almost-normal eye phenotypes. Half-dose reduction of the dMi-2 gene enhanced the DREF-induced rough-eye phenotype. Immunostaining of polytene chromosomes of salivary glands showed that dDREF and dMi-2 bind in mutually exclusive ways. These lines of evidence define a novel function of dMi-2 in the negative regulation of dDREF by its DNA-binding activity. Finally, we postulated that dDREF and dMi-2 may demonstrate reciprocal regulation of their functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.