ArticleMolecular and cellular biology2002
Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Emerging perspectives on multidomain phosphatidylinositol transfer proteins.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Review
- Reorganization of Ternary Lipid Mixtures of Nonphosphorylated Phosphatidylinositol Interacting with Angiomotin.The journal of physical chemistry. B · 2018Article
- Homeostatic regulation of the PI(4,5)P2-Ca(2+) signaling system at ER-PM junctions.Biochimica et biophysica acta · 2016Review
- Phosphatidylinositol and phosphatidic acid transport between the ER and plasma membrane during PLC activation requires the Nir2 protein.Biochemical Society transactions · 2016Review
- Loss of citron kinase affects a subset of progenitor cells that alters late but not early neurogenesis in the developing rat retina.Investigative ophthalmology & visual science · 2015Article
- Deficiency of the Cog8 subunit in normal and CDG-derived cells impairs the assembly of the COG and Golgi SNARE complexes.Traffic (Copenhagen, Denmark) · 2013Article
- Pitpnm1 is expressed in hair cells during development but is not required for hearing.Neuroscience · 2013Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.The Journal of cell biology · 2011Article
- The Golgi protein p115 associates with gamma-tubulin and plays a role in Golgi structure and mitosis progression.The Journal of biological chemistry · 2011Article
- Understanding cytokinesis failure.Advances in experimental medicine and biology · 2010Review
- Dual roles for the Drosophila PI 4-kinase four wheel drive in localizing Rab11 during cytokinesis.The Journal of cell biology · 2009Article
- Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.The EMBO journal · 2009Article
- Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis.Eukaryotic cell · 2007Article
- Regulation of cell cycle checkpoints by polo-like kinases.Oncogene · 2005Review
- Regulation of cell cycle by the anaphase spindle midzone.BMC cell biology · 2004Article
- Patellin1, a novel Sec14-like protein, localizes to the cell plate and binds phosphoinositides.Plant physiology · 2004Article
- Annexin 11 is required for midbody formation and completion of the terminal phase of cytokinesis.The Journal of cell biology · 2004Article
- A role for Arf1 in mitotic Golgi disassembly, chromosome segregation, and cytokinesis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinesis, the final stage of eukaryotic cell division, ensures the production of two daughter cells. It requires fine coordination between the plasma membrane and cytoskeletal networks, and it is known to be regulated by several intracellular proteins, including the small GTPase Rho and its effectors. In this study we provide evidence that the protein Nir2 is essential for cytokinesis. Microinjection of anti-Nir2 antibodies into interphase cells blocks cytokinesis, as it results in the production of multinucleate cells. Immunolocalization studies revealed that Nir2 is mainly localized in the Golgi apparatus in interphase cells, but it is recruited to the cleavage furrow and the midbody during cytokinesis. Nir2 colocalizes with the small GTPase RhoA in the cleavage furrow and the midbody, and it associates with RhoA in mitotic cells. Its N-terminal region, which contains a phosphatidylinositol transfer domain and a novel Rho-inhibitory domain (Rid), is required for normal cytokinesis, as overexpression of an N-terminal-truncated mutant blocks cytokinesis completion. Time-lapse videomicroscopy revealed that this mutant normally initiates cytokinesis but fails to complete it, due to cleavage furrow regression, while Rid markedly affects cytokinesis due to abnormal contractility. Rid-expressing cells exhibit aberrant ingression and ectopic cleavage sites; the cells fail to segregate into daughter cells and they form a long unseparated bridge-like cytoplasmic structure. These results provide new insight into the cellular functions of Nir2 and introduce it as a novel regulator of cytokinesis.
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