ArticleProceedings of the National Academy of Sciences of the United States of America2002
A ribozyme that ligates RNA to protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Supernova: A Deoxyribozyme that Catalyzes a Chemiluminescent Reaction.Angewandte Chemie (International ed. in English) · 2022Article
- Aptamers, Riboswitches, and Ribozymes inLife (Basel, Switzerland) · 2021Review
- Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020Article
- Catalytic Nucleic Acids: Biochemistry, Chemical Biology, Biosensors, and Nanotechnology.iScience · 2020Review
- Assessing histidine tags for recruiting deoxyribozymes to catalyze peptide and protein modification reactions.Organic & biomolecular chemistry · 2016Article
- Landmarks in the Evolution of (t)-RNAs from the Origin of Life up to Their Present Role in Human Cognition.Life (Basel, Switzerland) · 2015Review
- DNA-catalyzed lysine side chain modification.Angewandte Chemie (International ed. in English) · 2014Article
- A ribozyme that triphosphorylates RNA 5'-hydroxyl groups.Nucleic acids research · 2014Article
- Rapid RNA exchange in aqueous two-phase system and coacervate droplets.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2014Article
- Designing a bio-responsive robot from DNA origami.Journal of visualized experiments : JoVE · 2013Article
- Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription.RNA (New York, N.Y.) · 2013Article
- DNA-catalyzed reactivity of a phosphoramidate functional group and formation of an unusual pyrophosphoramidate linkage.Organic & biomolecular chemistry · 2012Article
- DNA-catalyzed covalent modification of amino acid side chains in tethered and free peptide substrates.Biochemistry · 2011Article
- Arginine cofactors on the polymerase ribozyme.PloS one · 2011Article
- An mRNA-protein fusion at N-terminus for evolutionary protein engineering.International journal of biological sciences · 2007Article
- Characterization of long RNA-cleaving deoxyribozymes with short catalytic cores: the effect of excess sequence elements on the outcome of in vitro selection.Nucleic acids research · 2006Article
- Evolutionary patterns of non-coding RNAs.Theory in biosciences = Theorie in den Biowissenschaften · 2005Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5' terminus of an RNA and a specific polypeptide. This reaction differs from that of previously identified ribozymes, although the product is analogous to the enzyme-nucleotidyl intermediates isolated during the reactions of certain proteinaceous enzymes, such as guanyltransferase, DNA ligase, and RNA ligase. Comparative sequence analysis of the isolated ribozymes revealed that they share a compact secondary structure containing six stems arranged in a four-helix junction and branched pseudoknot. An optimized version of the ribozyme reacts with substrate-fusion proteins, allowing it to be used to attach RNA tags to proteins both in vitro and within bacterial cells, suggesting a simple way to tag a specific protein with amplifiable information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.