ArticleProceedings of the National Academy of Sciences of the United States of America2002
Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 35 citations in OpenAlex.
- Mechanical properties of epithelial cells in domes investigated using atomic force microscopy.Frontiers in cell and developmental biology · 2023Article
- Review
- A Comparative Review of the Extrinsic and Intrinsic Factors Regulating Lactose Synthesis.Journal of mammary gland biology and neoplasia · 2021Review
- Cross-Species Meta-Analysis of Transcriptomic Data in Combination With Supervised Machine Learning Models Identifies the Common Gene Signature of Lactation Process.Frontiers in genetics · 2018Article
- Multipotent luminal mammary cancer stem cells model tumor heterogeneity.Breast cancer research : BCR · 2015Article
- SNORD-host RNA Zfas1 is a regulator of mammary development and a potential marker for breast cancer.RNA (New York, N.Y.) · 2011Article
- Activation of the aryl-hydrocarbon receptor inhibits invasive and metastatic features of human breast cancer cells and promotes breast cancer cell differentiation.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A rat mammary gland cancer cell with stem cell properties of self-renewal and multi-lineage differentiation.Cytotechnology · 2008Article
- The properties of a mammary gland cancer stem cell.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- kappa-casein-deficient mice fail to lactate.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Expression of the mouse fragilis gene products in immune cells and association with receptor signaling complexes.Genes and immunity · 2006Article
- Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Soluble extracts from Helicobacter pylori induce dome formation in polarized intestinal epithelial monolayers in a laminin-dependent manner.Infection and immunity · 2003Article
- Proteomic dissection of dome formation in a mammary cell line.Journal of mammary gland biology and neoplasia · 2002Review
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of the development of the mammary gland at the molecular level in the animal is difficult because of the complex tissue organization of the gland. We have previously developed an in vitro system for genetic analysis of mammary cell differentiation, based on the cell line LA7 clonally derived from a rat mammary adenocarcinoma. This cell line, after induction with DMSO, differentiates forming structures called domes. This process is under strict gene regulation, and we have previously identified several of the genes involved. In the present paper, we have defined the meaning of dome formation in relation to mammary development, by showing that treatment of LA7 cells with the lactogenic hormones hydrocortisone and prolactin induces dome formation; in the animal, these hormones precede and accompany milk production. Moreover, dome formation is accompanied by expression within the cells of the milk protein genes WDMN1 and beta-casein, which are differentiation markers for the gland during pregnancy and lactation. We also show that two proteins, highly expressed in the mammary gland during lactation, HSP90-beta and annexin I, are strongly expressed in DMSO-induced LA7 cells. Both proteins are essential in the formation of domes because when their synthesis is blocked by antisense RNA oligonucleotides, dome formation is abolished. Thus our in vitro system is a model for lobulo-alveolar development, and the genes identified in the pathway of dome formation are likely to be involved in the early differentiation steps occurring in the rat mammary gland during pregnancy and lactation.
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