ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Light response of retinal ON bipolar cells requires a specific splice variant of Galpha(o).
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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Who cites it
72 citing papers in PubMed, 123 citations in OpenAlex.
- Single-Cell Transcriptomics onBiomedicines · 2025Article
- Genetic Loss of RORα Impairs Visual Function With Rod Bipolar Cell Degeneration In Mice.Investigative ophthalmology & visual science · 2025Article
- GαJournal of neurochemistry · 2025Article
- Differential Localization and Functional Roles of mGluR6 Paralogs in Zebrafish Retina.Investigative ophthalmology & visual science · 2024Article
- Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice.iScience · 2023Article
- A visual opsin from jellyfish enables precise temporal control of G protein signalling.Nature communications · 2023Article
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- Bipolar cell targeted optogenetic gene therapy restores parallel retinal signaling and high-level vision in the degenerated retina.Communications biology · 2022Article
- Using a bistable animal opsin for switchable and scalable optogenetic inhibition of neurons.EMBO reports · 2021Article
- Arginyltransferase (Ate1) regulates the RGS7 protein level and the sensitivity of light-evoked ON-bipolar responses.Scientific reports · 2021Article
- Molecular mechanisms underlying selective synapse formation of vertebrate retinal photoreceptor cells.Cellular and molecular life sciences : CMLS · 2020Review
- The Cold Case of Metabotropic Glutamate Receptor 6: Unjust Detention in the Retina?Current neuropharmacology · 2020Review
- Transducin Partners Outside the Phototransduction Pathway.Frontiers in cellular neuroscience · 2020Article
- Differential epitope masking reveals synapse-specific complexes of TRPM1.Visual neuroscience · 2018Article
- Electrophysiological Studies on The Dynamics of Luminance Adaptation in the Mouse Retina.Vision (Basel, Switzerland) · 2017Article
- The Transduction Cascade in Retinal ON-Bipolar Cells: Signal Processing and Disease.Annual review of vision science · 2017Review
- Amyloid Precursor-Like Protein 2 deletion-induced retinal synaptopathy related to congenital stationary night blindness: structural, functional and molecular characteristics.Molecular brain · 2016Article
- Lack of mGluR6-related cascade elements leads to retrograde trans-synaptic effects on rod photoreceptor synapses via matrix-associated proteins.The European journal of neuroscience · 2016Article
- Biallelic Mutations in GNB3 Cause a Unique Form of Autosomal-Recessive Congenital Stationary Night Blindness.American journal of human genetics · 2016Article
- Full-field electroretinogram in autism spectrum disorder.Documenta ophthalmologica. Advances in ophthalmology · 2016Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Glutamate released onto retinal ON bipolar neurons binds to a metabotropic receptor to activate a heterotrimeric G-protein (G(o)) that ultimately closes a nonspecific cation channel. Signaling requires the alpha subunit (Galpha(o)), but its effector is unknown. Because Galpha(o) is transcribed into two splice variants (alpha(o1) and alpha(o2)) that differ in the key GTPase domain, the next step in elucidating this pathway was to determine which splice variant carries the signal. Here we show by reverse transcription-PCR and Western blots that retina expresses both splice variants. Furthermore, in situ hybridization and immunostaining on mouse retina deficient in one splice variant or the other show that both alpha(o1) and alpha(o2) are expressed by ON bipolar cells but that alpha(o1) is much more abundant. Finally, electroretinography performed on mice deficient for one splice variant or the other shows that the positive b-wave (response of ON bipolar cells to rod and cone input) requires alpha(o1) but not alpha(o2). Thus, the light response of the ON bipolar cell is probably carried by its strongly expressed splice variant, Galpha(o1).
Indexed as
Identifiers
12077185PMC6757744W2139672209What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.