ArticleGenetics2002
Efficient repair of DNA breaks in Drosophila: evidence for single-strand annealing and competition with other repair pathways.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Repeat mediated excision of gene drive elements for restoring wild-type populations.PLoS genetics · 2024Article
- Drosophila IRBP bZIP heterodimer binds P-element DNA and affects hybrid dysgenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- DNA Instability Maintains the Repeat Length of the Yeast RNA Polymerase II C-terminal Domain.The Journal of biological chemistry · 2016Article
- P Transposable Elements in Drosophila and other Eukaryotic Organisms.Microbiology spectrum · 2015Review
- DNA transposon-based gene vehicles - scenes from an evolutionary drive.Journal of biomedical science · 2013Review
- Condensin II subunit dCAP-D3 restricts retrotransposon mobilization in Drosophila somatic cells.PLoS genetics · 2013Article
- Germline excision of transgenes in Aedes aegypti by homing endonucleases.Scientific reports · 2013Article
- Mutation spectrum of Drosophila CNVs revealed by breakpoint sequencing.Genome biology · 2012Article
- Article
- DNA double-strand break repair and the evolution of intron density.Trends in genetics : TIG · 2011Article
- A new powerful method for site-specific transgene stabilization based on chromosomal double-strand break repair.PloS one · 2011Article
- Rad51 inhibits translocation formation by non-conservative homologous recombination in Saccharomyces cerevisiae.PloS one · 2010Article
- Drosophila bloom helicase maintains genome integrity by inhibiting recombination between divergent DNA sequences.Nucleic acids research · 2008Article
- A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Coincidence of P-insertion sites and breakpoints of deletions induced by activating P elements in Drosophila.Genetics · 2008Article
- Inviting instability: Transposable elements, double-strand breaks, and the maintenance of genome integrity.Mutation research · 2007Review
- DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase.Nucleic acids research · 2007Article
- Article
- Differential usage of alternative pathways of double-strand break repair in Drosophila.Genetics · 2006Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We show evidence that DNA double-strand breaks induced in the Drosophila germ line can be repaired very efficiently by the single-strand annealing (SSA) mechanism. A double-strand break was made between two copies of a 1290-bp direct repeat by mobilizing a P transposon. In >80% of the progeny that acquired this chromosome, repair resulted in loss of the P element and loss of one copy of the repeat, as observed in SSA. The frequency of this repair was much greater than seen for gene conversion using an allelic template, which is only approximately 7%. A similar structure, but with a smaller duplication of only 158 bp, also yielded SSA-like repair events, but at a reduced frequency, and gave rise to some products by repair pathways other than SSA. The 1290-bp repeats carried two sequence polymorphisms that were examined in the products. The allele nearest to a nick in the putative heteroduplex intermediate was lost most often. This bias is predicted by the SSA model, although other models could account for it. We conclude that SSA is the preferred repair pathway in Drosophila for DNA breaks between sequence repeats, and it competes with gene conversion by the synthesis-dependent strand annealing (SDSA) pathway.
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