ArticleGenetics2002
Volatile anesthetics affect nutrient availability in yeast.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- A comprehensive review and comparison of L-tryptophan biosynthesis inFrontiers in bioengineering and biotechnology · 2023Review
- Isoflurane induces Art2-Rsp5-dependent endocytosis of Bap2 in yeast.FEBS open bio · 2021Article
- Anesthetic pretreatment confers thermotolerance on Saccharomyces cerevisiae yeast.Biochemical and biophysical research communications · 2020Article
- Acetaminophen reduces the protein levels of high affinity amino acid permeases and causes tryptophan depletion.Amino acids · 2018Article
- Tetracaine, a local anesthetic, preferentially induces translational inhibition with processing body formation rather than phosphorylation of eIF2α in yeast.Current genetics · 2015Article
- Pressure-induced endocytic degradation of the Saccharomyces cerevisiae low-affinity tryptophan permease Tat1 is mediated by Rsp5 ubiquitin ligase and functionally redundant PPxY motif proteins.Eukaryotic cell · 2013Article
- Functional profiling discovers the dieldrin organochlorinated pesticide affects leucine availability in yeast.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Article
- Anesthetic action of volatile anesthetics by using Paramecium as a model.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2012Article
- Genome-wide analysis of tRNA charging and activation of the eIF2 kinase Gcn2p.The Journal of biological chemistry · 2009Article
- Membrane stress is coupled to a rapid translational control of gene expression in chlorpromazine-treated cells.Current genetics · 2007Article
- Antimicrobial effects of liquid anesthetic isoflurane on Candida albicans.Journal of translational medicine · 2006Article
- Inhibition of translation initiation by volatile anesthetics involves nutrient-sensitive GCN-independent and -dependent processes in yeast.Molecular biology of the cell · 2005Article
- Quantitative genome-wide analysis of yeast deletion strain sensitivities to oxidative and chemical stress.Comparative and functional genomics · 2004Article
- Pressure-induced differential regulation of the two tryptophan permeases Tat1 and Tat2 by ubiquitin ligase Rsp5 and its binding proteins, Bul1 and Bul2.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Volatile anesthetics affect all cells and tissues tested, but their mechanisms and sites of action remain unknown. To gain insight into the cellular activities of anesthetics, we have isolated genes that, when overexpressed, render Saccharomyces cerevisiae resistant to the volatile anesthetic isoflurane. One of these genes, WAK3/TAT1, encodes a permease that transports amino acids including leucine and tryptophan, for which our wild-type strain is auxotrophic. This suggests that availability of amino acids may play a key role in anesthetic response. Multiple lines of evidence support this proposal: (i) Deletion or overexpression of permeases that transport leucine and/or tryptophan alters anesthetic response; (ii) prototrophic strains are anesthetic resistant; (iii) altered concentrations of leucine and tryptophan in the medium affect anesthetic response; and (iv) uptake of leucine and tryptophan is inhibited during anesthetic exposure. Not all amino acids are critical for this response since we find that overexpression of the lysine permease does not affect anesthetic sensitivity. These findings are consistent with models in which anesthetics have a physiologically important effect on availability of specific amino acids by altering function of their permeases. In addition, we show that there is a relationship between nutrient availability and ubiquitin metabolism in this response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.