ArticleGenetics2002
Replication protein A is required for meiotic recombination in Saccharomyces cerevisiae.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
- Mei5-Sae3 stabilizes Dmc1 nucleating clusters for efficient Dmc1 assembly on RPA-coated single-stranded DNA.Nucleic acids research · 2024Article
- RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.Nucleic acids research · 2024Article
- Molecular mechanisms and regulation of recombination frequency and distribution in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast.PLoS genetics · 2023Article
- Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast.bioRxiv : the preprint server for biology · 2023Article
- Replication protein A large subunit (RPA1a) limits chiasma formation during rice meiosis.Plant physiology · 2021Article
- Meiotic Chromosome Dynamics in Zebrafish.Frontiers in cell and developmental biology · 2021Review
- Pathogenic Variations of Homologous Recombination GeneFrontiers in cell and developmental biology · 2021Article
- Sycp1 Is Not Required for Subtelomeric DNA Double-Strand Breaks but Is Required for Homologous Alignment in Zebrafish Spermatocytes.Frontiers in cell and developmental biology · 2021Article
- Meiotic Recombination Defects and Premature Ovarian Insufficiency.Frontiers in cell and developmental biology · 2021Review
- The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates.Molecular cell · 2020Article
- Dual functions for the ssDNA-binding protein RPA in meiotic recombination.PLoS genetics · 2019Article
- Meiocyte-Specific and AtSPO11-1-Dependent Small RNAs and Their Association with Meiotic Gene Expression and Recombination.The Plant cell · 2019Article
- Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in PolyploidFrontiers in plant science · 2017Article
- RPA homologs and ssDNA processing during meiotic recombination.Chromosoma · 2016Review
- Meiotic Recombination: The Essence of Heredity.Cold Spring Harbor perspectives in biology · 2015Review
- Replication protein A prevents promiscuous annealing between short sequence homologies: Implications for genome integrity.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- RPA antagonizes microhomology-mediated repair of DNA double-strand breaks.Nature structural & molecular biology · 2014Article
- Replication protein A2c coupled with replication protein A1c regulates crossover formation during meiosis in rice.The Plant cell · 2013Article
- Regulation of Deinococcus radiodurans RecA protein function via modulation of active and inactive nucleoprotein filament states.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In Saccharomyces cerevisiae, meiotic recombination is initiated by transient DNA double-stranded breaks (DSBs). These DSBs undergo a 5' --> 3' resection to produce 3' single-stranded DNA ends that serve to channel DSBs into the RAD52 recombinational repair pathway. In vitro studies strongly suggest that several proteins of this pathway--Rad51, Rad52, Rad54, Rad55, Rad57, and replication protein A (RPA)--play a role in the strand exchange reaction. Here, we report a study of the meiotic phenotypes conferred by two missense mutations affecting the largest subunit of RPA, which are localized in the protein interaction domain (rfa1-t11) and in the DNA-binding domain (rfa1-t48). We find that both mutant diploids exhibit reduced sporulation efficiency, very poor spore viability, and a 10- to 100-fold decrease in meiotic recombination. Physical analyses indicate that both mutants form normal levels of meiosis-specific DSBs and that the broken ends are processed into 3'-OH single-stranded tails, indicating that the RPA complex present in these rfa1 mutants is functional in the initial steps of meiotic recombination. However, the 5' ends of the broken fragments undergo extensive resection, similar to what is observed in rad51, rad52, rad55, and rad57 mutants, indicating that these RPA mutants are defective in the repair of the Spo11-dependent DSBs that initiate homologous recombination during meiosis.
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Registered trials
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