ArticleProceedings of the National Academy of Sciences of the United States of America2002
Liquid-liquid separation in solutions of normal and sickle cell hemoglobin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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Who cites it
52 citing papers in PubMed, 203 citations in OpenAlex.
- Navigating Classical and Nonclassical Realms of Nucleation and Crystallization.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Modeling Opposite Effects of an Additive on Liquid-Liquid Phase Separation and Crystal Solubility of Protein Solutions.Molecules (Basel, Switzerland) · 2026Article
- Controlled spherulitic crystal growth from salt mixtures.Communications chemistry · 2026Article
- The regulatory principles, physiological functions, and phase transition of biomolecular condensates.Frontiers in cell and developmental biology · 2026Review
- Liquid-liquid phase separation in normal hematopoiesis and hematological diseases.Cell and tissue research · 2025Review
- Liquid-liquid phase separation in cell physiology and cancer biology: recent advances and therapeutic implications.Frontiers in oncology · 2025Review
- Liquid-Liquid Phase Separation within Dense-Core Vesicles in Sympathetic Adrenal Chromaffin Cells.Neuroscience bulletin · 2024Article
- A dual growth mode unique for organic crystals relies on mesoscopic liquid precursors.Communications chemistry · 2024Article
- Article
- Effective interactions and phase behavior of protein solutions in the presence of hexamine cobalt(III) chloride.The European physical journal. E, Soft matter · 2023Article
- Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu.Nature communications · 2023Article
- Comprehensive Regulation of Liquid-Liquid Phase Separation of Polypeptides.Molecules (Basel, Switzerland) · 2023Article
- Identification of Phase-Separation-Protein-Related Function Based on Gene Ontology by Using Machine Learning Methods.Life (Basel, Switzerland) · 2023Article
- Rational Design of a Self-Assembling High Performance Organic Nanofluorophore for Intraoperative NIR-II Image-Guided Tumor Resection of Oral Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Phosphofructokinase relocalizes into subcellular compartments with liquid-like properties in vivo.Biophysical journal · 2021Article
- Mesoscopic protein-rich clusters host the nucleation of mutant p53 amyloid fibrils.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Water Loss in Aging Erythrocytes Provides a Clue to a General Mechanism of Cellular Senescence.Biophysical journal · 2020Review
- Atomic mechanism of metal crystal nucleus formation in a single-walled carbon nanotube.Nature chemistry · 2020Article
- Multivalent ions and biomolecules: Attempting a comprehensive perspective.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We show that in solutions of human hemoglobin (Hb)--oxy- and deoxy-Hb A or S--of near-physiological pH, ionic strength, and Hb concentration, liquid-liquid phase separation occurs reversibly and reproducibly at temperatures between 35 and 40 degrees C. In solutions of deoxy-HbS, we demonstrate that the dense liquid droplets facilitate the nucleation of HbS polymers, whose formation is the primary pathogenic event for sickle cell anemia. In view of recent results that shifts of the liquid-liquid separation phase boundary can be achieved by nontoxic additives at molar concentrations up to 30 times lower than the protein concentrations, these findings open new avenues for the inhibition of the HbS polymerization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.