ArticleAntimicrobial agents and chemotherapy2002
Inhibition of nanobacteria by antimicrobial drugs as measured by a modified microdilution method.
Article in Antimicrobial agents and chemotherapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Clinical implications of calcifying nanoparticles in dental diseases: a critical review.International journal of nanomedicine · 2014Pooled it
- Tetracycline attenuates calcifying nanoparticles-induced renal epithelial injury through suppression of inflammation, oxidative stress, and apoptosis in rat models.Translational andrology and urology · 2019Article
- Role of nanobacteria in the pathogenesis of kidney stone formation.American journal of translational research · 2016Article
- Resistance of nanobacteria isolated from urinary and kidney stones to broad-spectrum antibiotics.Iranian journal of microbiology · 2014Article
- An Environmental Risk Assessment for Human-Use Trimethoprim in European Surface Waters.Antibiotics (Basel, Switzerland) · 2013Article
- The role of calcifying nanoparticles in biology and medicine.International journal of nanomedicine · 2012Review
- Article
- Decreased nanobacteria levels and symptoms of nanobacteria-associated interstitial cystitis/painful bladder syndrome after tetracycline treatment.International urogynecology journal · 2010Article
- Characterization of biofilm formed by human-derived nanoparticles.Nanomedicine (London, England) · 2009Article
- Article
- Systemic injection of planktonic forms of mammalian-derived nanoparticles alters arterial response to injury in rabbits.American journal of physiology. Heart and circulatory physiology · 2009Article
- Putative nanobacteria represent physiological remnants and culture by-products of normal calcium homeostasis.PloS one · 2009Article
- Article
- The future of stone research: rummagings in the attic, Randall's plaque, nanobacteria, and lessons from phylogeny.Urological research · 2008Review
- Nanobacteria are mineralo fetuin complexes.PLoS pathogens · 2008Article
- Do blood-borne calcifying nanoparticles self-propagate?International journal of nanomedicine · 2008Article
- Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: proof of principle.International journal of nanomedicine · 2008Article
- Association between Randall's plaque and calcifying nanoparticles.International journal of nanomedicine · 2008Article
- Calcifying nanoparticles associated encrusted urinary bladder cystitis.International journal of nanomedicine · 2008Article
- An animal model of black pigment gallstones caused by nanobacteria.Digestive diseases and sciences · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compounds from 16 classes of antimicrobial drugs were tested for their abilities to inhibit the in vitro multiplication of nanobacteria (NB), a newly discovered infectious agent found in human kidney stones and kidney cyst fluids from patients with polycystic kidney disease (PKD). Because NB form surface calcifications at physiologic levels of calcium and phosphate, they have been hypothesized to mediate the formation of tissue calcifications. We describe a modified microdilution inhibitory test that accommodates the unique growth conditions and long multiplication times of NB. This modified microdilution method included inoculation of 96-well plates and determination of inhibition by periodic measurement of the absorbance for 14 days in cell culture medium under cell culture conditions. Bactericidal or bacteriostatic drug effects were distinguished by subsequent subculture in drug-free media and monitoring for increasing absorbance. NB isolated from fetal bovine serum (FBS) were inhibited by tetracycline HCl, nitrofurantoin, trimethoprim, trimethoprim-sulfamethoxazole, and ampicillin at levels achievable in serum and urine; all drugs except ampicillin were cidal. Tetracycline also inhibited multiplication of isolates of NB from human kidney stones and kidney cyst fluids from patients with PKD. The other antibiotics tested against FBS-derived NB either had no effect or exhibited an inhibitory concentration above clinically achievable levels; the aminoglycosides and vancomycin were bacteriostatic. Antibiotic-induced morphological changes to NB were observed by electron microscopy. Bisphosphonates, aminocaproic acid, potassium citrate-citric acid solutions, and 5-fluorouracil also inhibited the multiplication of NB in a cidal manner. Insights into the nature of NB, the action(s) of these drugs, and the role of NB in calcifying diseases may be gained by exploiting this in vitro inhibition test system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.