ArticlePharmaceutical research2002
Transfer of lipophilic markers from PLGA and polystyrene nanoparticles to caco-2 monolayers mimics particle uptake.
Article in Pharmaceutical research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 94 citations in OpenAlex.
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- Harnessing PET to track micro- and nanoplastics in vivo.Scientific reports · 2021Article
- Research on the fate of polymeric nanoparticles in the process of the intestinal absorption based on model nanoparticles with various characteristics: size, surface charge and pro-hydrophobics.Journal of nanobiotechnology · 2021Article
- Enhancing the Oral Bioavailability of Candesartan Cilexetil Loaded Nanostructured Lipid Carriers: In Vitro Characterization and Absorption in Rats after Oral Administration.Pharmaceutics · 2020Article
- Solid Lipid Nanoparticles Surface Modification Modulates Cell Internalization and Improves Chemotoxic Treatment in an Oral Carcinoma Cell Line.Nanomaterials (Basel, Switzerland) · 2019Article
- Transient loading of CD34International journal of nanomedicine · 2017Article
- Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers.Beilstein journal of nanotechnology · 2017Article
- Bidirectional Transfer Study of Polystyrene Nanoparticles across the Placental Barrier in an ex Vivo Human Placental Perfusion Model.Environmental health perspectives · 2015Article
- Transfer studies of polystyrene nanoparticles in theScience and technology of advanced materials · 2015Article
- Nanoparticle-mediated delivery of a rapidly activatable prodrug of SN-38 for neuroblastoma therapy.Biomaterials · 2015Article
- Cell localisation of gadolinium-based nanoparticles and related radiosensitising efficacy in glioblastoma cells.Cancer nanotechnology · 2014Article
- Models for oral uptake of nanoparticles in consumer products.Toxicology · 2012Review
- Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells.International journal of nanomedicine · 2011Article
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- Barrier capacity of human placenta for nanosized materials.Environmental health perspectives · 2010Article
- Self-assembled biodegradable nanoparticles developed by direct dialysis for the delivery of paclitaxel.Pharmaceutical research · 2005Article
- Intracellular drug delivery by poly(lactic-co-glycolic acid) nanoparticles, revisited.Molecular pharmaceuticsArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe objective of this study was to evaluate nanoparticle uptake by the Caco-2 monolayer model in vitro. Special emphasis was placed on the localization and the quantification of the uptake of fluorescently labeled polystyrene and poly(lactic-co-glycolic acid) (PLGA) nanoparticles.
methodsIntracellular fluorescence was localized by fluorescence and confocal laser scanning microscopy. Particle uptake was quantified either directly, by counting internalized nanoparticles after separation from the Caco-2 monolayers, or indirectly, by extraction of the lipophilic fluorescence marker. In vitro release studies of lipophilic markers from nanoparticles were performed in standard buffer systems and buffer systems supplemented with liposomes.
resultsInstead of uptake of polystyrene and PLGA nanoparticles by Caco-2 monolayers an efficient transfer of lipophilic fluorescence markers from nanoparticles into Caco-2 cells with subsequent staining of intracellular lipophilic compartments was observed. Whereas in standard buffer no release of fluorescent marker from polystyrene and PLGA nanoparticles was observed, the release studies using liposome dispersions as receiver revealed an efficient transfer of fluorescent marker into the liposome dispersion.
conclusionsThe results suggest that the deceptive particle uptake is caused by a collision-induced process facilitating the transfer of lipophilic fluorescent marker by formation of a complex between the nanoparticles and the biomembranes. Diffusion of the marker within this complex into lipophilic compartments of the cell strongly affects quantitative evaluation of particle uptake.
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