ArticleThe Journal of physiology2002
Intermittent ATP release from nerve terminals elicits focal smooth muscle Ca2+ transients in mouse vas deferens.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- Insights on Platelet-Derived Growth Factor Receptor α-Positive Interstitial Cells in the Male Reproductive Tract.International journal of molecular sciences · 2024Review
- Spinal cord injury alters purinergic neurotransmission to mesenteric arteries in rats.American journal of physiology. Heart and circulatory physiology · 2020Article
- Imaging sympathetic neurogenic CaAutonomic neuroscience : basic & clinical · 2017Review
- Nucleoside triphosphate diphosphohydrolase-1 ectonucleotidase is required for normal vas deferens contraction and male fertility through maintaining P2X1 receptor function.The Journal of biological chemistry · 2014Article
- Opposing roles of smooth muscle BK channels and ryanodine receptors in the regulation of nerve-evoked constriction of mesenteric resistance arteries.American journal of physiology. Heart and circulatory physiology · 2014Article
- A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function.Journal of muscle research and cell motility · 2012Article
- Prostaglandin E2 induces spontaneous rhythmic activity in mouse urinary bladder independently of efferent nerves.British journal of pharmacology · 2012Article
- Calcium signaling in smooth muscle.Cold Spring Harbor perspectives in biology · 2011Review
- Sympathetic overdrive in obesity involves purinergic hyperactivity in the resistance vasculature.The Journal of physiology · 2011Article
- Neuroeffector apparatus in gastrointestinal smooth muscle organs.The Journal of physiology · 2010Review
- Ca2+ imaging of activity in ICC-MY during local mucosal reflexes and the colonic migrating motor complex in the murine large intestine.The Journal of physiology · 2010Article
- Local elementary purinergic-induced Ca2+ transients: from optical mapping of nerve activity to local Ca2+ signaling networks.The Journal of general physiology · 2010Article
- Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate.British journal of pharmacology · 2010Article
- Nerve-evoked purinergic signalling suppresses action potentials, Ca2+ flashes and contractility evoked by muscarinic receptor activation in mouse urinary bladder smooth muscle.The Journal of physiology · 2009Article
- Focal Ca2+ transient detection in smooth muscle.Journal of visualized experiments : JoVE · 2009Article
- Neuroeffector Ca2+ transients for the direct measurement of purine release and indirect measurement of cotransmitters in rodents.Experimental physiology · 2009Article
- Spontaneous purinergic neurotransmission in the mouse urinary bladder.The Journal of physiology · 2008Article
- Smooth muscle cell calcium activation mechanisms.The Journal of physiology · 2008Review
- 5-HT4 receptor activation facilitates recovery from synaptic rundown and increases transmitter release from single varicosities of myenteric neurons.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- Bretylium abolishes neurotransmitter release without necessarily abolishing the nerve terminal action potential in sympathetic terminals.British journal of pharmacology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A confocal Ca2+ imaging technique has been used to detect ATP release from individual sympathetic varicosities on the same nerve terminal branch. Varicose nerve terminals and smooth muscle cells in mouse vas deferens were loaded with the Ca2+ indicator Oregon Green 488 BAPTA-1. Field (nerve) stimulation evoked discrete, focal increases in [Ca2+] in smooth muscle cells adjacent to identified varicosities. These focal increases in [Ca2+] have been termed 'neuroeffector Ca2+ transients' (NCTs). NCTs were abolished by alpha,beta-methylene ATP (1 microM), but not by nifedipine (1 microM) or prazosin (100 nM), suggesting that NCTs are generated by Ca2+ influx through P2X receptors without a detectable contribution from L-type Ca2+ channels or alpha(1)-adrenoceptor-mediated pathways. Action potential-evoked ATP release was highly intermittent (mean probability 0.019 +/- 0.002; range 0.001-0.10) at 1 Hz stimulation, even though there was no failure of action potential propagation in the nerve terminals. Twenty-eight per cent of varicosities failed to release transmitter following more than 500 stimuli. Spontaneous ATP release was very infrequent (0.0014 Hz). No Ca2+ transient attributable to noradrenaline release was detected even in response to 5 Hz stimulation. There was evidence of local noradrenaline release as the alpha(2)-adrenoceptor antagonist yohimbine increased the probability of occurrence of NCTs by 55 +/- 21 % during trains of stimuli at 1 Hz. Frequency-dependent facilitation preferentially occurred at low probability release sites. The monitoring of NCTs now allows transmitter release to be detected simultaneously from each functional varicosity on an identified nerve terminal branch on an impulse-to-impulse basis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.