ArticleThe Journal of physiology2002
Fast-to-slow transformation and nuclear import/export kinetics of the transcription factor NFATc1 during electrostimulation of rabbit muscle cells in culture.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Characteristics of the Protocols Used in Electrical Pulse Stimulation of Cultured Cells for Mimicking In Vivo Exercise: A Systematic Review, Meta-Analysis, and Meta-Regression.International journal of molecular sciences · 2022Pooled it
- Low-cost Devices for Stroke Rehabilitation: A Review of Approaches, Designs, and Evidence.Restorative neurology and neuroscience · 2026Review
- Molecular signaling effects of electrical stimulation of the soleus muscle during 6 days of dry immersion.Sports medicine and health science · 2026Article
- The Effect of SERCA Activation on Functional Characteristics and Signaling of Rat Soleus Muscle upon 7 Days of Unloading.Biomolecules · 2023Article
- Effects of Various Muscle Disuse States and Countermeasures on Muscle Molecular Signaling.International journal of molecular sciences · 2021Review
- Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease.Frontiers in physiology · 2021Review
- The role of in vivo Ca²⁺ signals acting on Ca²⁺-calmodulin-dependent proteins for skeletal muscle plasticity.The Journal of physiology · 2011Review
- Excitation-transcription coupling in skeletal muscle: the molecular pathways of exercise.Biological reviews of the Cambridge Philosophical Society · 2011Review
- Extracellular signal-regulated kinase 1/2-mediated phosphorylation of p300 enhances myosin heavy chain I/beta gene expression via acetylation of nuclear factor of activated T cells c1.Nucleic acids research · 2011Article
- Lineage-based primary muscle fiber type diversification independent of MEF2 and NFAT in chick embryos.Journal of muscle research and cell motility · 2011Article
- IP(3)-dependent, post-tetanic calcium transients induced by electrostimulation of adult skeletal muscle fibers.The Journal of general physiology · 2010Article
- Muscle fiber type specific induction of slow myosin heavy chain 2 gene expression by electrical stimulation.Experimental cell research · 2010Article
- [Exercise and cellular adaptation of muscle].Der Unfallchirurg · 2009Review
- The Ca(V) 1.2 Ca(2+) channel is expressed in sarcolemma of type I and IIa myofibers of adult skeletal muscle.Muscle & nerve · 2007Article
- Parallel mechanisms for resting nucleo-cytoplasmic shuttling and activity dependent translocation provide dual control of transcriptional regulators HDAC and NFAT in skeletal muscle fiber type plasticity.Journal of muscle research and cell motility · 2006Review
- Signaling pathways in activity-dependent fiber type plasticity in adult skeletal muscle.Journal of muscle research and cell motility · 2005Review
- NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Six1 and Eya1 expression can reprogram adult muscle from the slow-twitch phenotype into the fast-twitch phenotype.Molecular and cellular biology · 2004Article
- Pacing-induced calcineurin activation controls cardiac Ca2+ signalling and gene expression.The Journal of physiology · 2004Article
- Effects of electrostimulation on glycogenolysis in cultured rat myotubes.Pflugers Archiv : European journal of physiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contractile activity imposed by chronic electrical stimulation of a primary skeletal muscle cell culture grown on microcarriers over several days led to an increase of slow myosin heavy chain I (MHCI) and a decrease of fast MHCII expression at mRNA and protein levels, indicating an ongoing fast-to-slow transformation. Only patterns with periods of continuous stimulation of > or = 5 min in a 45 min cycle were capable of inducing a fibre type transformation, and this was independent of the applied stimulation frequency over the range 1-10 Hz. We have shown before that the calcineurin-NFATc1 signalling pathway is indispensable in mediating MHCI upregulation during fibre type transformation. Therefore, subcellular localization of NFATc1 was studied immunocytochemically. This revealed that only one stimulation train lasting for > or = 5 min was sufficient to induce nuclear import of this factor, which was about complete after 20 min of continuous stimulation. For both induction of NFATc1 import and MHCI mRNA upregulation, the minimum stimulation interval of > or = 5 min was sufficient and stimulation frequency was not crucial between 1 and 10 Hz. Repetition of stimulation cycles, with pauses (40 min) shorter than the time required for complete export of NFATc1, led to an accumulation of NFATc1 in the nuclei with each cycle and thus to an amplification of the transformation signal during extended periods of electrostimulation. The temporal behaviour of NFATc import/export appears to determine the effectiveness of various electrostimulation protocols in inducing fast-to-slow fibre transformation.
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