ArticleJournal of cellular and molecular medicine
Apoptosis in human embryo development: 3. Fas-induced apoptosis in brain primary cultures.
Article in Journal of cellular and molecular medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- Targeting the proliferation of glioblastoma cells and enhancement of doxorubicin and temozolomide cytotoxicity through inhibition of PFKFB4 and HMOX1 genes with siRNAs.Scientific reports · 2025Article
- The effects of harvesting media on biological characteristics and repair potential of neural stem cells after traumatic brain injury.PloS one · 2014Article
- Fas activation increases neural progenitor cell survival.Journal of neuroscience research · 2010Article
- Negative autoregulation by FAS mediates robust fetal erythropoiesis.PLoS biology · 2007Article
- Article
- Involvement of p53 and Fas/CD95 in murine neural progenitor cell response to ionizing irradiation.Oncogene · 2004Article
- Apoptosis in the immune system: 1. Fas-induced apoptosis in monocytes-derived human dendritic cells.Journal of cellular and molecular medicineArticle
- Phagocytosis of apoptotic cells by microglia in vitro.Journal of cellular and molecular medicineArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fas (APO-1/CD95) is an important apoptotic mediator for both immune and nervous systems. In the present study, we have investigated the expression and function of Fas in human embryonic/fetal brain primary cultures from 12 human embryos and fetuses with gestational ages between 5 to 22 weeks. Anti-Fas fluorescent antibody was used for labeling of Fas positive cells and for quantitation of Fas expression in brain cultures. To demonstrate that Fas receptor is functional in human embryonic/fetal brain cells, anti-Human-Fas monoclonal antibody (0.5 microg/ml) was used to induce apoptosis in brain primary cultures. Apoptosis was investigated by flow-cytometry and fluorescent microscopy using TUNEL and annexin V labeling. Fas was found to be expressed in the embryonic/fetal human primary brain cultures, on neuronal and glial cells or their precursors, varying with gestational ages. Cross-linking of Fas induced apoptosis in brain cultures indicating that Fas receptor functions as a death receptor. We also showed that cell death triggered through Fas receptor was caspase dependent, hence it was blocked by a selective caspase-8 inhibitor (IETD-fmk). These results suggest that Fas is involved in neuronal apoptosis in the developing human brain.
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