ArticleInfection and immunity2002
Macrophage-induced genes of Legionella pneumophila: protection from reactive intermediates and solute imbalance during intracellular growth.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Membrane Mimetics Control Internal Hydration and Catalytic Activity of the Copper ATPase LpCopA fromInternational journal of molecular sciences · 2026Article
- The structure-function analysis of Obg-like GTPase proteins along the evolutionary tree from bacteria to humans.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.Water research · 2017Article
- Intra-Species and Inter-Kingdom Signaling ofFrontiers in microbiology · 2017Review
- Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection.PLoS pathogens · 2015Article
- Involvement of a 1-Cys peroxiredoxin in bacterial virulence.PLoS pathogens · 2014Article
- The Legionella pneumophila kai operon is implicated in stress response and confers fitness in competitive environments.Environmental microbiology · 2014Article
- Reengineering redox sensitive GFP to measure mycothiol redox potential of Mycobacterium tuberculosis during infection.PLoS pathogens · 2014Article
- Induction of rapid cell death by an environmental isolate of Legionella pneumophila in mouse macrophages.Infection and immunity · 2013Article
- Tractable mammalian cell infections with protozoan-primed bacteria.Journal of visualized experiments : JoVE · 2013Article
- The Legionella pneumophila EnhC protein interferes with immunostimulatory muramyl peptide production to evade innate immunity.Cell host & microbe · 2012Article
- Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis.BMC microbiology · 2011Article
- Copper stress targets the rcs system to induce multiaggregative behavior in a copper-sensitive Salmonella strain.Journal of bacteriology · 2010Article
- Comparative study of the roles of AhpC and KatE as respiratory antioxidants in Brucella abortus 2308.Journal of bacteriology · 2010Article
- Copper homeostasis in Salmonella is atypical and copper-CueP is a major periplasmic metal complex.The Journal of biological chemistry · 2010Article
- A role for the ATP7A copper-transporting ATPase in macrophage bactericidal activity.The Journal of biological chemistry · 2009Article
- Legionella pneumophila EnhC is required for efficient replication in tumour necrosis factor alpha-stimulated macrophages.Cellular microbiology · 2008Article
- Significant role for ladC in initiation of Legionella pneumophila infection.Infection and immunity · 2008Article
- An ortholog of OxyR in Legionella pneumophila is expressed postexponentially and negatively regulates the alkyl hydroperoxide reductase (ahpC2D) operon.Journal of bacteriology · 2008Article
- Identification of in vivo-expressed immunogenic proteins by serological proteome analysis of the Bacillus anthracis secretome.Infection and immunity · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A promoter-probe strategy was devised to identify genes specifically expressed by Legionella pneumophila during growth within the macrophage. Random fragments from the L. pneumophila chromosome were inserted upstream of a promoterless phage T4 td gene, and fragments that led to complementation of thymine auxotrophy during intracellular growth of the bacterium were identified. Two different selection strategies were employed to eliminate promoters that were also active during extracellular growth of the bacterium. Some of these genes were identified independently by using both of the selection strategies. The factors identified include orthologs of efflux-mediated resistance determinants and transporters, a transporter involved in protection from osmotic stress, a stress response GTP-binding protein, a response regulator, a sensor kinase, and two systems that increase the reducing potential of the bacterium, one of which encodes the L. pneumophila ortholog of ahpC. Five of the clones analyzed here were fusions to promoters that were closely linked to genes encoding three-component chemiosmotic efflux pumps that export heavy metals or toxic organic compounds. Analysis of ahpC gene expression indicates that levels increased at least sevenfold during intracellular growth of the bacterium. Inactivation of several of the genes at their chromosomal loci had no effect on the intracellular growth rate of L. pneumophila in cultured macrophages. This suggests that a number of genes with increased expression during intracellular growth may be part of redundant systems that allow survival and growth under the conditions encountered within host cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.