ArticleInfection and immunity2002
Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 95 citations in OpenAlex.
- Repurposing epetraborole to combatAntimicrobial agents and chemotherapy · 2026Article
- Pathogenicity and virulence ofVirulence · 2025Review
- Study Models forMicroorganisms · 2025Review
- Dectin-1 stimulating β-glucans inhibit Chlamydia infections both in vitro and in vivo.Pathogens and disease · 2025Article
- Review
- Type III Secretion inMicrobiology and molecular biology reviews : MMBR · 2023Review
- The hormonal environment and estrogen receptor signaling altersFrontiers in cellular and infection microbiology · 2022Article
- Thiol-Mediated Uptake.JACS Au · 2021Review
- Rab Proteins: Insights into Intracellular Trafficking in Endometrium.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Incidence and progression of trachomatous scarring in a cohort of children in a formerly hyper-endemic district of Tanzania.PLoS neglected tropical diseases · 2020Article
- Organoid technology in female reproductive biomedicine.Reproductive biology and endocrinology : RB&E · 2020Review
- Article
- The complexity of interactions between female sex hormones andCurrent clinical microbiology reports · 2019Article
- Anti-chlamydia IgG and IgA are insufficient to prevent endometrial chlamydia infection in women, and increased anti-chlamydia IgG is associated with enhanced risk for incident infection.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Article
- Sulforaphane promotes chlamydial infection by suppressing mitochondrial protein oxidation and activation of complement C3.Protein science : a publication of the Protein Society · 2019Article
- The Chlamydia trachomatis PmpD adhesin forms higher order structures through disulphide-mediated covalent interactions.PloS one · 2018Article
- Chlamydiacae: Polymorphic membrane proteins make the difference.Virulence · 2016Article
- Article
- In vitro passage selects for Chlamydia muridarum with enhanced infectivity in cultured cells but attenuated pathogenicity in mouse upper genital tract.Infection and immunity · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydia trachomatis serovar E, the leading bacterial agent responsible for sexually transmitted diseases, is required to invade genital epithelial cells for its growth and survival, yet little is known about the adhesin-receptor interactions promoting its entry. In contrast, much has been published on the heparan sulfate receptor for binding C. trachomatis L2 elementary bodies (EBs) prior to entry into HeLa cells. Using a different experimental approach in which a biotinylated apical membrane protein receptor(s) attached to EB at 4 degrees C was stripped off the surface of polarized HEC-1B cells and immunoprecipitated with polyclonal anti-EB antibodies, an approximately 55-kDa protein was reproducibly detected by enhanced chemiluminescence and two-dimensional gel electrophoresis. Matrix-assisted laser desorption ionization mass-spectrometry sequence analysis revealed the 55-kDa protein to be protein disulfide isomerase (PDI), a member of the estrogen receptor complex which carries out thiol-disulfide exchange reactions at infected host cell surfaces. Exposure of HEC-1B cells during EB attachment (1.5 to 2 h) to three different inhibitors of PDI reductive reactions--(i) the thiol-alkylating reagent DTNB (5,5'-dithiobis[2-nitrobenzoic acid]), (ii) bacitracin, and (iii) anti-PDI antibodies--resulted in reduced chlamydial infectivity. Since (i) C. trachomatis serovar E attachment to estrogen-dominant primary human endometrial epithelial cells is dramatically enhanced and (ii) productive entry into and infectivity of EB in host cells is dependent on reduction of EB cross-linked outer membrane proteins at the host cell surface, these data provide some preliminary evidence for an intriguing new potential receptor candidate for further analysis of luminal C. trachomatis serovar E entry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.