ArticleInfection and immunity2002
Identification of Escherichia coli genes that are specifically expressed in a murine model of septicemic infection.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Novel Virulence Factors DecipheringInternational journal of molecular sciences · 2022Article
- Crystal Structure of the YcjX Stress Protein Reveals a Ras-Like GTP-Binding Protein.Journal of molecular biology · 2019Article
- Synthetic Gene Circuits Enable Systems-Level Biosensor Trigger Discovery at the Host-Microbe Interface.mSystems · 2019Article
- Comparative analysis of Klebsiella pneumoniae genomes identifies a phospholipase D family protein as a novel virulence factor.BMC biology · 2014Article
- Genomic diversity and adaptation of Salmonella enterica serovar Typhimurium from analysis of six genomes of different phage types.BMC genomics · 2013Article
- Identification of Avian pathogenic Escherichia coli genes that are induced in vivo during infection in chickens.Applied and environmental microbiology · 2012Article
- Genome-wide transcriptional response of an avian pathogenic Escherichia coli (APEC) pst mutant.BMC genomics · 2008Article
- Escherichia coli O157:H7 survives within human macrophages: global gene expression profile and involvement of the Shiga toxins.Infection and immunity · 2008Article
- Identification of a Novel Regulator for the Escherichia coli fit Iron Transport System.The open microbiology journal · 2008Article
- Identification of candidates for a subunit vaccine against extraintestinal pathogenic Escherichia coli.Infection and immunity · 2007Article
- Identification and characterization of a novel ABC iron transport system, fit, in Escherichia coli.Infection and immunity · 2006Article
- Inactivation of the pst system reduces the virulence of an avian pathogenic Escherichia coli O78 strain.Infection and immunity · 2005Article
- Analysis of Pseudomonas putida KT2440 gene expression in the maize rhizosphere: in vivo [corrected] expression technology capture and identification of root-activated promoters.Journal of bacteriology · 2005Article
- Unraveling the secret lives of bacteria: use of in vivo expression technology and differential fluorescence induction promoter traps as tools for exploring niche-specific gene expression.Microbiology and molecular biology reviews : MMBR · 2005Review
- RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis.Journal of bacteriology · 2004Article
- Identification of Lactobacillus plantarum genes that are induced in the gastrointestinal tract of mice.Journal of bacteriology · 2004Article
- Pathogenic Escherichia coli.Nature reviews. Microbiology · 2004Review
- Genomic scale analysis of Pasteurella multocida gene expression during growth within the natural chicken host.Infection and immunity · 2002Article
- In vivo expression technology.Infection and immunity · 2002Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification and characterization of bacterial genes that are induced during the disease process are important in understanding the molecular mechanism of disease and can be useful in designing antimicrobial drugs to control the disease. The identification of in vivo induced (ivi) genes of an Escherichia coli septicemia strain by using antibiotic-based in vivo expression technology is described. Bacterial clones resistant to chloramphenicol in vivo were recovered from the livers of infected mice. Most of the ivi clones were sensitive to chloramphenicol when grown in vitro. Using reverse transcription-PCR, it was demonstrated that selected ivi clones expressed cat in the livers of infected mice but not during in vitro growth. A total of 750 colonies were recovered after three successive rounds of in vivo selection, and 168 isolated ivi clones were sequenced. The sequence analysis revealed that 37 clones encoded hypothetical proteins found in E. coli K-12, whereas 10 clones contained genes that had no significant homology to DNA sequences in GenBank. Two clones were found to contain transposon-related functions. Other clones contained genes required for amino acid metabolism, anaerobic respiration, DNA repair, the heat shock response, and the cellular repressor of the SOS response. In addition, one clone contained the aerobactin biosynthesis gene iucA. Mutations were introduced in to seven of the identified ivi genes. An in vivo mouse challenge-competition assay was used to determine if the mutants were attenuated. The results suggested that these ivi genes were important for survival in vivo, and three of the seven mutant ivi clones were required for successful infection of mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.