ArticleThe EMBO journal2002
Shigella deliver an effector protein to trigger host microtubule destabilization, which promotes Rac1 activity and efficient bacterial internalization.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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Who cites it
40 citing papers in PubMed, 119 citations in OpenAlex.
- Article
- Evidence for intracellularJournal of bacteriology · 2024Review
- Microtubules provide force to promote membrane uncoating in vacuolar escape for a cyto-invasive bacterial pathogen.Nature communications · 2024Article
- Article
- NOD-Like Receptors: Guards of Cellular Homeostasis Perturbation during Infection.International journal of molecular sciences · 2021Review
- Using proteomics to identify host cell interaction partners for VgrG and IglJ.Scientific reports · 2020Article
- Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens.International journal of molecular sciences · 2020Review
- Article
- Recent insights into Shigella.Current opinion in infectious diseases · 2018Review
- Tracking elusive cargo: Illuminating spatio-temporal Type 3 effector protein dynamics using reporters.Cellular microbiology · 2018Review
- How Do the Virulence Factors ofFrontiers in cellular and infection microbiology · 2017Review
- Manipulation of host membranes by the bacterial pathogens Listeria, Francisella, Shigella and Yersinia.Seminars in cell & developmental biology · 2016Review
- The genomic signatures of Shigella evolution, adaptation and geographical spread.Nature reviews. Microbiology · 2016Review
- Selective protection of an ARF1-GTP signaling axis by a bacterial scaffold induces bidirectional trafficking arrest.Cell reports · 2014Article
- Recent advances in understanding enteric pathogenic Escherichia coli.Clinical microbiology reviews · 2013Review
- Actin cytoskeleton manipulation by effector proteins secreted by diarrheagenic Escherichia coli pathotypes.BioMed research international · 2013Review
- Modulation of host microtubule dynamics by pathogenic bacteria.Biomolecular concepts · 2012Article
- Structural and functional studies indicate that the EPEC effector, EspG, directly binds p21-activated kinase.Biochemistry · 2011Article
- EseG, an effector of the type III secretion system of Edwardsiella tarda, triggers microtubule destabilization.Infection and immunity · 2010Article
- Unifying themes in microbial associations with animal and plant hosts described using the gene ontology.Microbiology and molecular biology reviews : MMBR · 2010Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella deliver a subset of effectors into the host cell via the type III secretion system, that stimulate host cell signal pathways to modulate the actin dynamics required for invasion of epithelial cells. Here we show that one of the Shigella effectors, called VirA, can interact with tubulin to promote microtubule (MT) destabilization, and elicit protrusions of membrane ruffling. Under in vitro conditions, VirA inhibited polymerization of tubulin and stimulated MT destabilization. Upon microinjection of VirA into HeLa cells, a localized membrane ruffling was induced rapidly. Overexpression of VirA in host cells caused MT destruction and protruding membrane ruffles which were absent when VirA was co-expressed with a dominant-negative Rac1 mutant. Indeed, Shigella but not the virA mutant stimulated Rac1, including the formation of membrane ruffles in infected cells. Importantly, the MT structure beneath the protruding ruffling was destroyed. Furthermore, drug-induced MT growth in HeLa cells greatly enhanced the Shigella entry. These results indicate that VirA is a novel type of bacterial effector capable of inducing membrane ruffling through the stimulation of MT destabilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.