ArticleProceedings of the National Academy of Sciences of the United States of America2002
Rapid genetic analysis of Helicobacter pylori gastric mucosal colonization in suckling mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Establishment of an intragastric surgical model using C57BL/6 mice to study the vaccine efficacy of OMV-based immunogens against Helicobacter pylori.Biology open · 2025Article
- Copper Efflux System Required in Murine Lung Infection by Haemophilus influenzae Composed of a Canonical ATPase Gene and Tandem Chaperone Gene Copies.Infection and immunity · 2023Article
- Enhanced Fitness of a Helicobacter pyloriInfection and immunity · 2021Article
- Gastric Mucosa-Associated Microbial Signatures of Early Gastric Cancer.Frontiers in microbiology · 2020Article
- Identification of the RNA Pyrophosphohydrolase RppH of Helicobacter pylori and Global Analysis of Its RNA Targets.The Journal of biological chemistry · 2017Article
- To be or not to be: The host genetic factor and beyond in Helicobacter pylori mediated gastro-duodenal diseases.World journal of gastroenterology · 2015Review
- An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Role of innate immunity in Helicobacter pylori-induced gastric malignancy.Physiological reviews · 2010Review
- Surreptitious manipulation of the human host by Helicobacter pylori.Gut microbes · 2010Article
- Antibody-mediated protection against infection with Helicobacter pylori in a suckling mouse model of passive immunity.Infection and immunity · 2009Article
- Helicobacter pylori in health and disease.Gastroenterology · 2009Review
- Expanding the Helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector.Applied and environmental microbiology · 2007Article
- Identification of Helicobacter pylori genes that contribute to stomach colonization.Infection and immunity · 2007Article
- Helicobacter pylori: present status and future prospects in Japan.Journal of gastroenterology · 2007Review
- Overview: Helicobacter pylori and extragastric disease.International journal of hematology · 2006Review
- Helicobacter pylori persistence: an overview of interactions between H. pylori and host immune defenses.Clinical microbiology reviews · 2006Review
- Helicobacter pylori initiates the stringent response upon nutrient and pH downshift.Journal of bacteriology · 2006Article
- Going against the grain: chemotaxis and infection in Vibrio cholerae.Nature reviews. Microbiology · 2005Review
- Development of an interleukin-12-deficient mouse model that is permissive for colonization by a motile KE26695 strain of Helicobacter pylori.Infection and immunity · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previously described animal models for Helicobacter pylori infection have been limited by cumbersome host requirements (e.g., germ-free conditions or unusual species) or are applicable to only special subsets of H. pylori strains (e.g., fresh clinical isolates or animal-adapted derivatives). Here, we report that 5- to 6-day-old outbred CD-1 (ICR) suckling mice support 24-h colonization of all H. pylori strains tested (SS1, 26695 SmR-1, 43504 SmR-1, and G27 SmR-1), including lab-passaged strains that cannot be adapted for colonization of adult animals. Total colony-forming units (cfu) recovered from infection with lab-passaged strains did not differ from those with mouse-adapted SS1. We also tested this model's ability to detect colonization defects in strains carrying mutations in known virulence genes by coinfecting with wild-type H. pylori and measuring differential recovery. This competition assay identified colonization defects in several classes of known attenuated mutants, including those defective in acid resistance (ureA), metabolism (frdA), motility (motB), and chemotaxis (cheY). A mutant defective in copA (copper transporting P-type ATPase) is nonattenuated in adult and infant mice. Possibly because of the limited duration of infection, our model did not identify defects in vacuolating cytotoxin (vacA) or gamma-glutamyltranspeptidase (ggt) as attenuating, in contrast to results from other animal models. We also identified a new virulence gene (HP0507) encoding a conserved hypothetical protein, which is important for colonization in our model. The suckling mouse model offers a rapid method to identify colonization defects in any H. pylori strain and may have utility as a new tool for studying immunity to primary infection.
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