ArticleNucleic acids research2002
DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales.Communications biology · 2025Article
- Single-Molecule Methods for Investigating the Double-Stranded DNA Bendability.Molecules and cells · 2022Review
- Lysine Methylation Modulates the Interaction of Archaeal Chromatin Protein Cren7 With DNA.Frontiers in microbiology · 2022Article
- Archaea: A Gold Mine for Topoisomerase Diversity.Frontiers in microbiology · 2021Review
- InMicroorganisms · 2020Article
- Article
- Methylation deficiency of chromatin proteins is a non-mutational and epigenetic-like trait in evolved lines of the archaeonThe Journal of biological chemistry · 2019Article
- In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag.PloS one · 2017Article
- Sulfolobus Spindle-Shaped Virus 1 Contains Glycosylated Capsid Proteins, a Cellular Chromatin Protein, and Host-Derived Lipids.Journal of virology · 2015Article
- Structural and sequencing analysis of local target DNA recognition by MLV integrase.Nucleic acids research · 2015Article
- Insights into the interaction between Cren7 and DNA: the role of loop β3-β4.Extremophiles : life under extreme conditions · 2015Article
- DNA recognition by a σ(54) transcriptional activator from Aquifex aeolicus.Journal of molecular biology · 2014Article
- Chromatin structure and dynamics in hot environments: architectural proteins and DNA topoisomerases of thermophilic archaea.International journal of molecular sciences · 2014Review
- Insight into the cellular involvement of the two reverse gyrases from the hyperthermophilic archaeon Sulfolobus solfataricus.BMC molecular biology · 2014Article
- Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.Extremophiles : life under extreme conditions · 2014Review
- The reverse gyrase from Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities.The Journal of biological chemistry · 2014Article
- Sulfolobus chromatin proteins modulate strand displacement by DNA polymerase B1.Nucleic acids research · 2013Article
- A prototypic lysine methyltransferase 4 from archaea with degenerate sequence specificity methylates chromatin proteins Sul7d and Cren7 in different patterns.The Journal of biological chemistry · 2013Article
- Sa-Lrp from Sulfolobus acidocaldarius is a versatile, glutamine-responsive, and architectural transcriptional regulator.MicrobiologyOpen · 2013Article
- Crenarchaeal chromatin proteins Cren7 and Sul7 compact DNA by inducing rigid bends.Nucleic acids research · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the Sso7d/Sac7d family are small, abundant, non-specific DNA-binding proteins of the hyperthermophilic Archaea SULFOLOBUS: Crystal structures of these proteins in complex with oligonucleotides showed that they induce changes in the helical twist and marked DNA bending. On this basis they have been suggested to play a role in organising chromatin structures in these prokaryotes, which lack histones. We report functional in vitro assays to investigate the effects of the observed Sso7d-induced structural modifications on DNA geometry and topology. We show that binding of multiple Sso7d molecules to short DNA fragments induces significant curvature and reduces the stiffness of the complex. Sso7d induces negative supercoiling of DNA molecules of any topology (relaxed, positively or negatively supercoiled) and in physiological conditions of temperature and template topology. Binding of Sso7d induces compaction of positively supercoiled and relaxed DNA molecules, but not of negatively supercoiled ones. Finally, Sso7d inhibits the positive supercoiling activity of the thermophile-specific enzyme reverse gyrase. The proposed biological relevance of these observations is that these proteins might model the behaviour of DNA in constrained chromatin environments.
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