ArticleMolecular biology of the cell2002
Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Regulation of Pfh1 helicase activity by nucleic acid interactions and mitochondrial SSB.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The increase in cell death rates in caloric restricted cells of the yeast helicase mutant rrm3 is Sir complex dependent.Scientific reports · 2023Article
- Role and Regulation of Pif1 Family Helicases at the Replication Fork.International journal of molecular sciences · 2022Review
- Pif1 family DNA helicases: A helpmate to RNase H?DNA repair · 2019Review
- TheGenetics · 2019Article
- Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast.PLoS genetics · 2019Article
- Article
- The functions of the multi-tasking Pfh1Current genetics · 2017Review
- Review
- G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase.Nucleic acids research · 2016Article
- Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding.Nucleic acids research · 2016Article
- The Pif1 family helicase Pfh1 facilitates telomere replication and has an RPA-dependent role during telomere lengthening.DNA repair · 2014Article
- Human PIF1 helicase supports DNA replication and cell growth under oncogenic-stress.Oncotarget · 2014Article
- To peep into Pif1 helicase: multifaceted all the way from genome stability to repair-associated DNA synthesis.Journal of microbiology (Seoul, Korea) · 2014Review
- DNA repair at telomeres: keeping the ends intact.Cold Spring Harbor perspectives in biology · 2013Review
- Regulation of telomere addition at DNA double-strand breaks.Chromosoma · 2013Review
- DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.Genes & development · 2012Article
- A genomewide screen for suppressors of Alu-mediated rearrangements reveals a role for PIF1.PloS one · 2012Article
- The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis.BMC molecular biology · 2011Article
- Telomeres: structures in need of unwinding.FEBS letters · 2010Review
Corrections and comments
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6 authors.
Funding
Abstract
The Saccharomyces cerevisiae Pif1p DNA helicase is the prototype member of a helicase subfamily conserved from yeast to humans. S. cerevisiae has two PIF1-like genes, PIF1 itself and RRM3, that have roles in maintenance of telomeric, ribosomal, and mitochondrial DNA. Here we describe the isolation and characterization of pfh1+, a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. Pfh1p was the only S. pombe protein with high identity to Saccharomyces Pif1p. Unlike the two S. cerevisiae Pif1 subfamily proteins, the S. pombe Pfh1p was essential. Like Saccharomyces Pif1p, a truncated form of the S. pombe protein had 5' to 3' DNA helicase activity. Point mutations in an invariant lysine residue in the ATP binding pocket of Pfh1p had the same phenotype as deleting pfh1+, demonstrating that the ATPase/helicase activity of Pfh1p was essential. Although mutant spores depleted for Pfh1p proceeded through S phase, they arrested with a terminal cellular phenotype consistent with a postinitiation defect in DNA replication. Telomeric DNA was modestly shortened in the absence of Pfh1p. However, genetic analysis demonstrated that maintenance of telomeric DNA was not the sole essential function of S. pombe Pfh1p.
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