ArticleMolecular biology of the cell2002
Stu1p is physically associated with beta-tubulin and is required for structural integrity of the mitotic spindle.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Kinetochore clustering is mediated by Mps1 phosphorylation of conserved MELT motifs in Stu1.The Journal of cell biology · 2026Article
- Kinetochore clustering is mediated by Mps1 phosphorylation of conserved MELT motifs in Stu1.bioRxiv : the preprint server for biology · 2026Article
- Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression inInternational journal of molecular sciences · 2025Article
- TheCells · 2025Article
- Plasticity of the mitotic spindle in response to karyotype variation.Current biology : CB · 2024Article
- MAPping tubulin mutations.Frontiers in cell and developmental biology · 2023Review
- Genome-wide identification and characterization of the CLASP_N gene family in upland cotton (PeerJ · 2022Article
- Slk19 enhances cross-linking of microtubules by Ase1 and Stu1.Molecular biology of the cell · 2021Article
- Chemical rescue of mutant proteins in living Saccharomyces cerevisiae cells by naturally occurring small molecules.G3 (Bethesda, Md.) · 2021Article
- Review
- CLASPs at a glance.Journal of cell science · 2020Review
- Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans.PLoS genetics · 2019Article
- Human CLASP2 specifically regulates microtubule catastrophe and rescue.Molecular biology of the cell · 2018Article
- Unattached kinetochores drive their own capturing by sequestering a CLASP.Nature communications · 2018Article
- Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules.The Journal of cell biology · 2017Article
- A TOGL domain specifically targets yeast CLASP to kinetochores to stabilize kinetochore microtubules.The Journal of cell biology · 2014Article
- A cryptic TOG domain with a distinct architecture underlies CLASP-dependent bipolar spindle formation.Structure (London, England : 1993) · 2013Article
- Mitotic spindle form and function.Genetics · 2012Article
- Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8.The Journal of cell biology · 2010Article
- CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule.Developmental cell · 2010Article
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Authors and funding
5 authors.
Funding
Abstract
Formation of the bipolar mitotic spindle relies on a balance of forces acting on the spindle poles. The primary outward force is generated by the kinesin-related proteins of the BimC family that cross-link antiparallel interpolar microtubules and slide them past each other. Here, we provide evidence that Stu1p is also required for the production of this outward force in the yeast Saccharomyces cerevisiae. In the temperature-sensitive stu1-5 mutant, spindle pole separation is inhibited, and preanaphase spindles collapse, with their previously separated poles being drawn together. The temperature sensitivity of stu1-5 can be suppressed by doubling the dosage of Cin8p, a yeast BimC kinesin-related protein. Stu1p was observed to be a component of the mitotic spindle localizing to the midregion of anaphase spindles. It also binds to microtubules in vitro, and we have examined the nature of this interaction. We show that Stu1p interacts specifically with beta-tubulin and identify the domains required for this interaction on both Stu1p and beta-tubulin. Taken together, these findings suggest that Stu1p binds to interpolar microtubules of the mitotic spindle and plays an essential role in their ability to provide an outward force on the spindle poles.
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