ArticleMolecular biology of the cell2002
Centrosome reorientation in wound-edge cells is cell type specific.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
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- Golgi Stabilization, Not Its Front-Rear Bias, Is Associated with EMT-Enhanced Fibrillar Migration.Biophysical journal · 2018Article
- Multicompartment cell-based modeling of confined migration: regulation by cell intrinsic and extrinsic factors.Molecular biology of the cell · 2018Article
- Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules.eLife · 2018Article
- Centrosome defines the rear of cells during mesenchymal migration.Molecular biology of the cell · 2017Article
- Polarity Reversal by Centrosome Repositioning Primes Cell Scattering during Epithelial-to-Mesenchymal Transition.Developmental cell · 2017Article
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- Fat-Dachsous signaling coordinates cartilage differentiation and polarity during craniofacial development.PLoS genetics · 2014Article
- Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia.Journal of neuroscience methods · 2014Article
- Tight coupling between nucleus and cell migration through the perinuclear actin cap.Journal of cell science · 2014Article
- Miro-1 links mitochondria and microtubule Dynein motors to control lymphocyte migration and polarity.Molecular and cellular biology · 2014Article
- Cellular polarity in aging: role of redox regulation and nutrition.Genes & nutrition · 2014Article
- Cep192 controls the balance of centrosome and non-centrosomal microtubules during interphase.PloS one · 2014Article
- Mitochondrial localization and the persistent migration of epithelial cancer cells.Biophysical journal · 2013Article
- The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.Cell cycle (Georgetown, Tex.) · 2013Article
- Improved-throughput traction microscopy based on fluorescence micropattern for manual microscopy.PloS one · 2013Article
- Centrosome positioning in vertebrate development.Journal of cell science · 2012Review
- Orientation and function of the nuclear-centrosomal axis during cell migration.Current opinion in cell biology · 2011Review
- Orientation and polarity in collectively migrating cell structures: statics and dynamics.Biophysical journal · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The reorientation of the microtubule organizing center during cell migration into a wound in the monolayer was directly observed in living wound-edge cells expressing gamma-tubulin tagged with green fluorescent protein. Our results demonstrate that in CHO cells, the centrosome reorients to a position in front of the nucleus, toward the wound edge, whereas in PtK cells, the centrosome lags behind the nucleus during migration into the wound. In CHO cells, the average rate of centrosome motion was faster than that of the nucleus; the converse was true in PtK cells. In both cell lines, centrosome motion was stochastic, with periods of rapid motion interspersed with periods of slower motion. Centrosome reorientation in CHO cells required dynamic microtubules and cytoplasmic dynein/dynactin activity and could be prevented by altering cell-to-cell or cell-to-substrate adhesion. Microtubule marking experiments using photoactivation of caged tubulin demonstrate that microtubules are transported in the direction of cell motility in both cell lines but that in PtK cells, microtubules move individually, whereas their movement is more coherent in CHO cells. Our data demonstrate that centrosome reorientation is not required for directed migration and that diverse cells use distinct mechanisms for remodeling the microtubule array during directed migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.