ArticleMolecular biology of the cell2002
The cytoplasmic tail of invariant chain regulates endosome fusion and morphology.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- De novo formation of early endosomes during Rab5-to-Rab7a transition.Journal of cell science · 2021Article
- Endosomal binding kinetics of Eps15 and Hrs specifically regulate the degradation of RTKs.Scientific reports · 2017Article
- Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.Molecular biology of the cell · 2015Article
- Exposing the Specific Roles of the Invariant Chain Isoforms in Shaping the MHC Class II Peptidome.Frontiers in immunology · 2013Review
- The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain.The Journal of experimental medicine · 2013Article
- Towards a systems understanding of MHC class I and MHC class II antigen presentation.Nature reviews. Immunology · 2011Review
- Critical role for invariant chain in CD1d-mediated selection and maturation of Vα14-invariant NKT cells.Immunology letters · 2011Article
- Requirement for invariant chain in macrophages for Mycobacterium tuberculosis replication and CD1d antigen presentation.Infection and immunity · 2011Article
- Stoichiometry of HLA class II-invariant chain oligomers.PloS one · 2011Article
- Endolysosomal proteases and their inhibitors in immunity.Nature reviews. Immunology · 2009Review
- Cell-cycle-dependent binding kinetics for the early endosomal tethering factor EEA1.EMBO reports · 2008Article
- Role of the major histocompatibility complex class II transmembrane region in antigen presentation and intracellular trafficking.Immunology · 2004Article
- PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or sorting of procathepsin D by regulating multivesicular body morphogenesis.Molecular biology of the cell · 2003Article
- Innate self recognition by an invariant, rearranged T-cell receptor and its immune consequences.Immunology · 2003Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major histocompatibility complex class II associated invariant chain (Ii) has been shown to inhibit endocytic transport and to increase the size of endosomes. We have recently found that this property has a significant impact on antigen processing and presentation. Here, we show in a cell-free endosome fusion assay that expression of Ii can increase fusion after phosphatidylinositol 3-kinase activity is blocked by wortmannin. In live cells wortmannin was also not able to block formation of the Ii-induced enlarged endosomes. The effects of Ii on endosomal transport and morphology depend on elements within the cytoplasmic tail. Data from mutagenesis analysis and nuclear magnetic resonance-based structure calculations of the Ii cytoplasmic tail demonstrate that free negative charges that are not involved in internal salt bridges are essential for both interactions between the tails and for the formation of enlarged endosomes. This correlation indicates that it is interactions between the Ii cytoplasmic tails that are involved in endosome fusion. The combined data from live cells, cell-free assays, and molecular dynamic simulations suggest that Ii molecules on different vesicles can promote endosome docking and fusion and thereby control endosomal traffic of membrane proteins and endosomal content.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.