ArticleMolecular biology of the cell2002
Kinesin-like protein CHO1 is required for the formation of midbody matrix and the completion of cytokinesis in mammalian cells.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 147 citations in OpenAlex.
- Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system.iScience · 2026Article
- KIF23 regulates cytoskeleton dynamics for cytokinesis and mitochondrial function during oocyte meiosis.Cell communication and signaling : CCS · 2026Article
- Extracellular vesicles, including large translating vesicles called midbody remnants, are released during the cell cycle.Molecular biology of the cell · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.The Journal of cell biology · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.bioRxiv : the preprint server for biology · 2023Article
- The mammalian midbody and midbody remnant are assembly sites for RNA and localized translation.Developmental cell · 2023Article
- Article
- CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.Communications biology · 2021Article
- The kinesin-like protein Pavarotti functions noncanonically to regulate actin dynamics.The Journal of cell biology · 2020Article
- Revealing PAK2's Function in the Cell Division through MKLP1's Interactome.BioMed research international · 2020Article
- Review
- Kinesin 6 Regulation inG3 (Bethesda, Md.) · 2018Article
- Kinesin-6 KIF20B is required for efficient cytokinetic furrowing and timely abscission in human cells.Molecular biology of the cell · 2018Article
- Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6.Molecular biology of the cell · 2017Article
- Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.Molecular biology of the cell · 2016Article
- CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation.PLoS biology · 2015Article
- Phosphorylation of CHO1 by Lats1/2 regulates the centrosomal activation of LIMK1 during cytokinesis.Cell cycle (Georgetown, Tex.) · 2015Article
- The chromosomal passenger complex (CPC) as a key orchestrator of orderly mitotic exit and cytokinesis.Frontiers in cell and developmental biology · 2015Review
- SHCBP1 is required for midbody organization and cytokinesis completion.Cell cycle (Georgetown, Tex.) · 2014Article
- Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
CHO1 is a mammalian kinesin-like motor protein of the MKLP1 subfamily. It associates with the spindle midzone during anaphase and concentrates to a midbody matrix during cytokinesis. CHO1 was originally implicated in karyokinesis, but the invertebrate homologues of CHO1 were shown to function in the midzone formation and cytokinesis. To analyze the role of the protein in mammalian cells, we mutated the ATP-binding site of CHO1 and expressed it in CHO cells. Mutant protein (CHO1F') was able to interact with microtubules via ATP-independent microtubule-binding site(s) but failed to accumulate at the midline of the central spindle and affected the localization of endogenous CHO1. Although the segregation of chromosomes, the bundling of midzone microtubules, and the initiation of cytokinesis proceeded normally in CHO1F'-expressing cells, the completion of cytokinesis was inhibited. Daughter cells were frequently entering interphase while connected by a microtubule-containing cytoplasmic bridge from which the dense midbody matrix was missing. Depletion of endogenous CHO1 via RNA-mediated interference also affected the formation of midbody matrix in dividing cells, caused the disorganization of midzone microtubules, and resulted in abortive cytokinesis. Thus, CHO1 may not be required for karyokinesis, but it is essential for the proper midzone/midbody formation and cytokinesis in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.