ArticleJournal of bacteriology2002
Functional interaction of region 4 of the extracytoplasmic function sigma factor FecI with the cytoplasmic portion of the FecR transmembrane protein of the Escherichia coli ferric citrate transport system.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- A simple and unified protocol to purify all seven Escherichia coli RNA polymerase sigma factors.Journal of applied genetics · 2024Article
- Transcription regulation of iron carrier transport genes by ECF sigma factors through signaling from the cell surface into the cytoplasm.FEMS microbiology reviews · 2022Article
- Towards the complete proteinaceous regulome ofMicrobial genomics · 2017Article
- Identification and characterization of Cronobacter iron acquisition systems.Applied and environmental microbiology · 2012Article
- Structural and biochemical bases for the redox sensitivity of Mycobacterium tuberculosis RslA.Journal of molecular biology · 2010Article
- A Novel extracytoplasmic function (ECF) sigma factor regulates virulence in Pseudomonas aeruginosa.PLoS pathogens · 2009Article
- Signaling mechanisms for activation of extracytoplasmic function (ECF) sigma factors.Biochimica et biophysica acta · 2008Review
- Signal transduction pathway of TonB-dependent transporters.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The heterologous siderophores ferrioxamine B and ferrichrome activate signaling pathways in Pseudomonas aeruginosa.Journal of bacteriology · 2006Article
- Iron and Pseudomonas aeruginosa biofilm formation.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- FpvIR control of fpvA ferric pyoverdine receptor gene expression in Pseudomonas aeruginosa: demonstration of an interaction between FpvI and FpvR and identification of mutations in each compromising this interaction.Journal of bacteriology · 2005Article
- Sites of interaction between the FecA and FecR signal transduction proteins of ferric citrate transport in Escherichia coli K-12.Journal of bacteriology · 2003Article
- Lessons from the genome of a lithoautotroph: making biomass from almost nothing.Journal of bacteriology · 2003Article
- Analysis of the ferric citrate transport gene promoter of Escherichia coli.Journal of bacteriology · 2003Article
- The FecI extracytoplasmic-function sigma factor of Escherichia coli interacts with the beta' subunit of RNA polymerase.Journal of bacteriology · 2003Article
- Heme-responsive transcriptional activation of Bordetella bhu genes.Journal of bacteriology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional regulation of the ferric citrate transport genes of Escherichia coli is initiated by the binding of ferric citrate to the outer membrane protein FecA. This binding elicits a signal that is transmitted by FecR across the cytoplasmic membrane into the cytoplasm, where the sigma factor FecI directs the RNA polymerase to the promoter upstream of the fecABCDE genes. An in vivo deletion analysis using a bacterial two-hybrid system assigned the interaction of the FecR and FecI proteins to the cytoplasmic portion of the FecR transmembrane protein and region 4 of FecI. Missense mutations randomly generated by PCR were localized to region 4 of FecI, and the mutants were impaired with regard to the interaction of FecR with FecI and fecB-lacZ transcription. The cloned region 4 of FecI interfered with fecB-lacZ transcription. Interaction of N-proximal regions of predicted FecR homologs with region 4 of predicted FecI homologs of Pseudomonas aeruginosa was demonstrated. The interaction was specific in that only cognate protein pairs interacted with each other; no interactions occurred between heterologous combinations of the P. aeruginosa proteins and between a P. aeruginosa FecI homolog and E. coli FecR. The results demonstrate that region 4 of FecI specifically binds FecR and that this binding is necessary for FecI to function as a sigma factor.
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