ArticleJournal of bacteriology2002
Involvement of the PrrB/PrrA two-component system in nitrite respiration in Rhodobacter sphaeroides 2.4.3: evidence for transcriptional regulation.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Transcriptional and Post-transcriptional Control of the Nitrate Respiration in Bacteria.Frontiers in molecular biosciences · 2021Review
- Mapping of the Denitrification Pathway in Burkholderia thailandensis by Genome-Wide Mutant Profiling.Journal of bacteriology · 2020Article
- The Global Redox Responding RegB/RegA Signal Transduction System Regulates the Genes Involved in Ferrous Iron and Inorganic Sulfur Compound Oxidation of the AcidophilicFrontiers in microbiology · 2017Article
- RegA Plays a Key Role in Oxygen-Dependent Establishment of Persistence and in Isocitrate Lyase Activity, a Critical Determinant ofFrontiers in cellular and infection microbiology · 2017Article
- Article
- The flagellar set Fla2 in Rhodobacter sphaeroides is controlled by the CckA pathway and is repressed by organic acids and the expression of Fla1.Journal of bacteriology · 2015Article
- The global response regulator RegR controls expression of denitrification genes in Bradyrhizobium japonicum.PloS one · 2014Article
- RegA, the regulator of the two-component system RegB/RegA of Brucella suis, is a controller of both oxidative respiration and denitrification required for chronic infection in mice.Infection and immunity · 2013Article
- Bacterial adaptation of respiration from oxic to microoxic and anoxic conditions: redox control.Antioxidants & redox signaling · 2012Review
- Physiological roles for two periplasmic nitrate reductases in Rhodobacter sphaeroides 2.4.3 (ATCC 17025).Journal of bacteriology · 2011Article
- Identification, functional studies, and genomic comparisons of new members of the NnrR regulon in Rhodobacter sphaeroides.Journal of bacteriology · 2010Article
- Agrobacterium tumefaciens C58 uses ActR and FnrN to control nirK and nor expression.Journal of bacteriology · 2008Article
- Dominant role of the cbb3 oxidase in regulation of photosynthesis gene expression through the PrrBA system in Rhodobacter sphaeroides 2.4.1.Journal of bacteriology · 2007Article
- Activation of the global gene regulator PrrA (RegA) from Rhodobacter sphaeroides.Biochemistry · 2006Article
- Assessing the impact of denitrifier-produced nitric oxide on other bacteria.Applied and environmental microbiology · 2006Article
- Expression of nitrite and nitric oxide reductases in free-living and plant-associated Agrobacterium tumefaciens C58 cells.Applied and environmental microbiology · 2005Article
- Regulation and function of cytochrome c' in Rhodobacter sphaeroides 2.4.3.Journal of bacteriology · 2005Article
- Denitrification genes regulate Brucella virulence in mice.Journal of bacteriology · 2004Article
- RegB/RegA, a highly conserved redox-responding global two-component regulatory system.Microbiology and molecular biology reviews : MMBR · 2004Review
- Use of a green fluorescent protein-based reporter fusion for detection of nitric oxide produced by denitrifiers.Applied and environmental microbiology · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhodobacter sphaeroides strain 2.4.3 is capable of diverse metabolic lifestyles, including denitrification. The regulation of many Rhodobacter genes involved in redox processes is controlled, in part, by the PrrBA two-component sensor-regulator system, where PrrB serves as the sensor kinase and PrrA is the response regulator. Four strains of 2.4.3 carrying mutations within the prrB gene were isolated in a screen for mutants unable to grow anaerobically on medium containing nitrite. Studies revealed that the expression of nirK, the structural gene encoding nitrite reductase, in these strains was significantly decreased compared to its expression in 2.4.3. Disruption of prrA also eliminated the ability to grow both photosynthetically and anaerobically in the dark on nitrite-amended medium. Complementation with prrA restored the wild-type phenotype. The PrrA strain exhibited a severe decrease in both nitrite reductase activity and expression of a nirK-lacZ fusion. Nitrite reductase activity in the PrrA strain could be restored to wild-type levels by using nirK expressed from a heterologous promoter, suggesting that the loss of nitrite reductase activity in the PrrA and PrrB mutants was not due to problems with enzyme assembly or the supply of reductant. Inactivation of prrA had no effect on the expression of the gene encoding NnrR, a transcriptional activator required for the expression of nirK. Inactivation of ccoN, part of the cbb(3)-type cytochrome oxidase shown to regulate the kinase activity of PrrB, also caused a significant decrease in both nirK expression and Nir activity. This was unexpected, since PrrA-P accumulates in the ccoN strain. Together, these results demonstrate that PrrBA plays an essential role in the regulation of nirK.
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