ArticleMolecular and cellular biology2002
Two ras pathways in fission yeast are differentially regulated by two ras guanine nucleotide exchange factors.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
- Constitutively active RAS prolongs Cdc42 signalling, while MAPK signalling is attenuated during fission yeast mating.PLoS genetics · 2026Article
- Rax1/2 promote memory of growth by early Ras1 activation to maintain polarity across generations.bioRxiv : the preprint server for biology · 2025Article
- RAS signalling genes can be used as host-induced gene silencing targets to control fungal diseases caused by Sclerotinia sclerotiorum and Botrytis cinerea.Plant biotechnology journal · 2024Article
- The Devastating Rice Blast Airborne PathogenPathogens (Basel, Switzerland) · 2023Review
- Pheromone Response and Mating Behavior in Fission Yeast.Microbiology and molecular biology reviews : MMBR · 2022Review
- Sporulation: A response to starvation in the fission yeast Schizosaccharomyces pombe.MicrobiologyOpen · 2022Review
- Cdc42 reactivation at growth sites is regulated by local cell-cycle-dependent loss of its GTPase-activating protein Rga4 in fission yeast.Journal of cell science · 2021Article
- Article
- Conserved NDR/LATS kinase controls RAS GTPase activity to regulate cell growth and chronological lifespan.Molecular biology of the cell · 2019Article
- SH3-class Ras guanine nucleotide exchange factors are essential for Aspergillus fumigatus invasive growth.Cellular microbiology · 2019Article
- Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.Journal of cell science · 2018Article
- Exploration and stabilization of Ras1 mating zone: A mechanism with positive and negative feedbacks.PLoS computational biology · 2018Article
- Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating.The Journal of cell biology · 2018Article
- RasGRF Couples Nox4-Dependent Endoplasmic Reticulum Signaling to Ras.Arteriosclerosis, thrombosis, and vascular biology · 2017Article
- Article
- Control of Ras-mediated signaling in Aspergillus fumigatus.Mycopathologia · 2014Review
- Beyond symmetry-breaking: competition and negative feedback in GTPase regulation.Trends in cell biology · 2013Review
- FYPO: the fission yeast phenotype ontology.Bioinformatics (Oxford, England) · 2013Article
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
How a given Ras prreotein coordinates multiple signaling inputs and outputs is a fundamental issue of signaling specificity. Schizosaccharomyces pombe contains one Ras, Ras1, that has two distinct outputs. Ras1 activates Scd1, a presumptive guanine nucleotide exchange factor (GEF) for Cdc42, to control morphogenesis and chromosome segregation, and Byr2, a component of a mitogen-activated protein kinase cascade, to control mating. So far there is only one established Ras1 GEF, Ste6. Paradoxically, ste6 null (ste6 Delta) mutants are sterile but normal in cell morphology. This suggests that Ste6 specifically activates the Ras1-Byr2 pathway and that there is another GEF capable of activating the Scd1 pathway. We thereby characterized a potential GEF, Efc25. Genetic data place Efc25 upstream of the Ras1-Scd1, but not the Ras1-Byr2, pathway. Like ras1 Delta and scd1 Delta, efc25 Delta is synthetically lethal with a deletion in tea1, a critical element for cell polarity control. Using truncated proteins, we showed that the C-terminal GEF domain of Efc25 is essential for function and regulated by the N terminus. We conclude that Efc25 acts as a Ras1 GEF specific for the Scd1 pathway. While ste6 expression is induced during mating, efc25 expression is constitutive. Moreover, Efc25 overexpression renders cells hyperelongated and sterile; the latter can be rescued by activated Ras1. This suggests that Efc25 can recruit Ras1 to selectively activate Scd1 at the expense of Byr2. Reciprocally, Ste6 overexpression can block Scd1 activation. We propose that external signals can partly segregate two Ras1 pathways by modulating GEF expression and that GEFs can influence how Ras is coupled to specific effectors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.