ArticleBMC musculoskeletal disorders2002
Recruitment, augmentation and apoptosis of rat osteoclasts in 1,25-(OH)2D3 response to short-term treatment with 1,25-dihydroxyvitamin D3 in vivo.
Article in BMC musculoskeletal disorders, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Liraglutide in Combination with Insulin Has a Superior Therapeutic Effect to Either Alone on Fracture Healing in Diabetic Rats.Diabetes, metabolic syndrome and obesity : targets and therapy · 2023Article
- Role of the Myokine Irisin on Bone Homeostasis: Review of the Current Evidence.International journal of molecular sciences · 2021Review
- A soluble bone morphogenetic protein type 1A receptor fusion protein treatment prevents glucocorticoid-Induced bone loss in mice.American journal of translational research · 2019Article
- Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence.International journal of biological sciences · 2019Article
- Strontium Ranelate Combined with Insulin Is as Beneficial as Insulin Alone in Treatment of Fracture Healing in Ovariectomized Diabetic Rats.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- CBX7 deficiency plays a positive role in dentin and alveolar bone development.Journal of molecular histology · 2016Article
- Overexpression of Bmi1 in Lymphocytes Stimulates Skeletogenesis by Improving the Osteogenic Microenvironment.Scientific reports · 2016Article
- CYP24 inhibition as a therapeutic target in FGF23-mediated renal phosphate wasting disorders.The Journal of clinical investigation · 2016Article
- Synergistic effects of high dietary calcium and exogenous parathyroid hormone in promoting osteoblastic bone formation in mice.The British journal of nutrition · 2015Article
- Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells.BioMed research international · 2015Review
- Recombinant human parathyroid hormone related protein 1-34 and 1-84 and their roles in osteoporosis treatment.PloS one · 2014Article
- Black tea may be a prospective adjunct for calcium supplementation to prevent early menopausal bone loss in a rat model of osteoporosis.Journal of osteoporosis · 2013Article
- Article
- Endogenous PTH deficiency impairs fracture healing and impedes the fracture-healing efficacy of exogenous PTH(1-34).PloS one · 2011Article
- Protective Role of Black Tea Extract against Nonalcoholic Steatohepatitis-Induced Skeletal Dysfunction.Journal of osteoporosis · 2011Article
- Comparison of active vitamin D compounds and a calcimimetic in mineral homeostasis.Journal of the American Society of Nephrology : JASN · 2010Article
- Alterations in phosphorus, calcium and PTHrP contribute to defects in dental and dental alveolar bone formation in calcium-sensing receptor-deficient mice.Development (Cambridge, England) · 2010Article
- Evidence-based assessment of antiosteoporotic activity of petroleum-ether extract of Cissus quadrangularis Linn. on ovariectomy-induced osteoporosis.Upsala journal of medical sciences · 2009Article
- HCO3-/Cl- anion exchanger SLC4A2 is required for proper osteoclast differentiation and function.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough much is known about the regulation of osteoclast (OC) formation and activity, little is known about OC senescence. In particular, the fate of of OC seen after 1,25-(OH)2D3 administration in vivo is unclear. There is evidence that the normal fate of OC is to undergo apoptosis (programmed cell death). We have investigated the effect of short-term application of high dose 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on OC apoptosis in an experimental rat model.
methodsOC recruitment, augmentation and apoptosis was visualised and quantitated by staining histochemically for tartrate resistant acid phosphatase (TRAP), double staining for TRAP/ED1 or TRAP/DAPI, in situ DNA fragmentation end labelling and histomorphometric analysis.
resultsShort-term treatment with high-dose 1,25-(OH)2D3 increased the recruitment of OC precursors in the bone marrow resulting in a short-lived increase in OC numbers. This was rapidly followed by an increase in the number of apoptotic OC and their subsequent removal. The response of OC to 1,25-(OH)2D3 treatment was dose and site dependent; higher doses producing stronger, more rapid responses and the response in the tibiae being consistently stronger and more rapid than in the vertebrae.
conclusionsThis study demonstrates that (1) after recruitment, OC are removed from the resorption site by apoptosis (2) the combined use of TRAP and ED1 can be used to identify OC and their precursors in vivo (3) double staining for TRAP and DAPI or in situ DNA fragmentation end labelling can be used to identify apoptotic OC in vivo.
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Registered trials
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