ArticleBreast cancer research : BCR2002
Gene expression profiles derived from fine needle aspiration correlate with response to systemic chemotherapy in breast cancer.
Article in Breast cancer research : BCR, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 219 citations in OpenAlex.
- Large-scale meta-analysis of cancer microarray data identifies common transcriptional profiles of neoplastic transformation and progression.Proceedings of the National Academy of Sciences of the United States of America · 2004Pooled it
- The effect of the stromal component of breast tumours on prediction of clinical outcome using gene expression microarray analysis.Breast cancer research : BCR · 2006Trial
- Genetic Signature of Breast Cancer Defines Therapeutic Interventions and Indicates Early Drug Resistance.Journal of cellular and molecular medicine · 2025Review
- Awareness and current knowledge of breast cancer.Biological research · 2017Review
- Molecular and cellular heterogeneity in breast cancer: challenges for personalized medicine.The American journal of pathology · 2013Review
- Molecular profiling of thin-prep FNA samples in assisting clinical management of non-small-cell lung cancer.Molecular biotechnology · 2013Article
- Assessment of fine needle aspiration feasibility and specimen adequacy for molecular diagnostics of benign vocal fold lesions.The Laryngoscope · 2013Article
- Novel functional assay for spindle-assembly checkpoint by cyclin-dependent kinase activity to predict taxane chemosensitivity in breast tumor patient.Journal of Cancer · 2013Article
- Clinical and multiple gene expression variables in survival analysis of breast cancer: analysis with the hypertabastic survival model.BMC medical genomics · 2012Article
- High-throughput molecular analysis from leftover of fine needle aspiration cytology of mammographically detected breast cancer.Translational oncology · 2012Article
- Development of an eight gene expression profile implicating human breast tumours of all grade.Molecular biology reports · 2012Article
- Genomic instability in breast and ovarian cancers: translation into clinical predictive biomarkers.Cellular and molecular life sciences : CMLS · 2012Review
- Alteration of cellular behavior and response to PI3K pathway inhibition by culture in 3D collagen gels.PloS one · 2012Article
- Apoptosis - associated genes and their role in predicting responses to neoadjuvant breast cancer treatment.Medical science monitor : international medical journal of experimental and clinical research · 2012Article
- Global array-based transcriptomics from minimal input RNA utilising an optimal RNA isolation process combined with SPIA cDNA probes.PloS one · 2011Article
- Fine-needle aspiration biopsies for gene expression ratio-based diagnostic and prognostic tests in malignant pleural mesothelioma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2011Article
- Gene-expression profiling in breast cancer: bespoke cancer therapy or more fiction than science?Irish journal of medical science · 2010Article
- Reliable gene expression measurements from fine needle aspirates of pancreatic tumors: effect of amplicon length and quality assessment.The Journal of molecular diagnostics : JMD · 2010Article
- Article
- HOXA9 regulates BRCA1 expression to modulate human breast tumor phenotype.The Journal of clinical investigation · 2010Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDrug resistance in breast cancer is a major obstacle to successful chemotherapy. In this study we used cDNA microarray technology to examine gene expression profiles obtained from fine needle aspiration (FNA) of primary breast tumors before and after systemic chemotherapy. Our goal was to determine the feasibility of obtaining representative expression array profiles from limited amounts of tissue and to identify those expression profiles that correlate with treatment response.
methodsRepeat presurgical FNA samples were taken from six patients who were to undergo primary surgical treatment. Additionally, a group of 10 patients who were to receive neoadjuvant chemotherapy underwent two FNAs before chemotherapy (adriamycin 60 mg/m2 and cyclophosphamide 600 mg/m2) followed by another FNA on day 21 after the first cycle. Total RNA was amplified with T7 Eberwine's procedure and labeled cDNA was hybridized onto a 7600-feature glass cDNA microarray.
resultsWe identified candidate gene expression profiles that might distinguish tumors with complete response to chemotherapy from tumors that do not respond, and found that the number of genes that change after one cycle of chemotherapy was 10 times greater in the responding group than in the non-responding group.
conclusionThis study supports the suitability of FNA-derived cDNA microarray expression profiling of breast cancers as a comprehensive genomic approach for studying the mechanisms of drug resistance. Our findings also demonstrate the potential of monitoring post-chemotherapy changes in expression profiles as a measure of pharmacodynamic effect and suggests that these approaches might yield useful results when validated by larger studies.
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