ArticleAnnals of biomedical engineering2002
Intracellular calcium changes in rat aortic smooth muscle cells in response to fluid flow.
Article in Annals of biomedical engineering, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Spontaneous Helical Alignment of Smooth Muscle Cells to Form a Medial Layer for Engineered Microvasculature.Advanced healthcare materials · 2026Article
- Interstitial flow, pressure and residual stress in the aging carotid artery model in FEBio.Biomechanics and modeling in mechanobiology · 2024Article
- Remote and Selective Control of Astrocytes by Magnetomechanical Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Vascular smooth muscle cell glycocalyx mediates shear stress-induced contractile responses via a Rho kinase (ROCK)-myosin light chain phosphatase (MLCP) pathway.Scientific reports · 2017Article
- Complex hemodynamic insult in combination with wall degeneration at the apex of an arterial bifurcation contributes to generation of nascent aneurysms in a canine model.AJNR. American journal of neuroradiology · 2014Article
- Flow shear stress enhances intracellular Ca2+ signaling in pulmonary artery smooth muscle cells from patients with pulmonary arterial hypertension.American journal of physiology. Cell physiology · 2014Article
- Fluid Mechanics, Arterial Disease, and Gene Expression.Annual review of fluid mechanics · 2014Article
- Involvement of large conductance Ca(2+)-activated K (+) channel in laminar shear stress-induced inhibition of vascular smooth muscle cell proliferation.Pflugers Archiv : European journal of physiology · 2013Article
- Altered hemodynamics, endothelial function, and protein expression occur with aortic coarctation and persist after repair.American journal of physiology. Heart and circulatory physiology · 2012Article
- Mechanosensory calcium signaling.Advances in experimental medicine and biology · 2012Review
- Two distinct phases of calcium signalling under flow.Cardiovascular research · 2011Article
- Fluid flow mechanotransduction in vascular smooth muscle cells and fibroblasts.Annals of biomedical engineering · 2011Review
- Enhanced interstitial flow as a contributing factor in neointima formation: (shear) stressing vascular wall cell types other than the endothelium.American journal of physiology. Heart and circulatory physiology · 2009Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Vascular smooth muscle cells (VSM) are normally exposed to transmural fluid flow shear stresses, and after vascular injury, blood flow shear stresses are imposed upon them. Since Ca2+ is a ubiquitous intracellular signaling molecule, we examined the effects of fluid flow on intracellular Ca2+ concentration in rat aortic smooth muscle cells to assess VSM responsiveness to shear stress. Cells loaded with fura 2 were exposed to steady flow shear stress levels of 0.5-10.0 dyn/cm2 in a parallel-plate flow chamber. The percentage of cells displaying a rise in cytosolic Ca2+ ion concentration ([Ca2+]i) increased in response to increasing flow, but there was no effect of flow on the ([Ca2+]i) amplitude of responding cells. Addition of Gd3+ (10 microM) or thapsigargin (50 nM) significantly reduced the percentage of cells responding and the response amplitude, suggesting that influx of Ca2+ through ion channels and release from intracellular stores contribute to the rise in ([Ca2+]i) in response to flow. The addition of nifedipine (1 or 10 microM) or ryanodine (10 microM) also significantly reduced the response amplitude, further defining the role of ion channels and intracellular stores in the Ca2+ response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.