ArticleAnnals of biomedical engineering2002
In vitro characterization and micromechanics of tumor cell chemotactic protrusion, locomotion, and extravasation.
Article in Annals of biomedical engineering, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
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- Immunoediting of leukocyte functions within the tumor microenvironment promotes cancer metastasis development.Biorheology · 2009Article
- Integrin VLA-4 enhances sialyl-Lewisx/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions.American journal of physiology. Cell physiology · 2008Article
- The tumor cell-host organ interface in the early onset of metastatic organ colonisation.Clinical & experimental metastasis · 2008Review
- Stepping out of the flow: capillary extravasation in cancer metastasis.Clinical & experimental metastasis · 2008Review
- Targeting mutant (V600E) B-Raf in melanoma interrupts immunoediting of leukocyte functions and melanoma extravasation.Cancer research · 2007Article
- Regulation of interleukin-8 expression in melanoma-stimulated neutrophil inflammatory response.Experimental cell research · 2007Article
- Targeting mitogen-activated protein kinase/extracellular signal-regulated kinase kinase in the mutant (V600E) B-Raf signaling cascade effectively inhibits melanoma lung metastases.Cancer research · 2006Article
- Melanoma cell extravasation under flow conditions is modulated by leukocytes and endogenously produced interleukin 8.Molecular & cellular biomechanics : MCB · 2005Article
- Distinct role of hydrodynamic shear in leukocyte-facilitated tumor cell extravasation.American journal of physiology. Cell physiology · 2005Article
- Neutrophils influence melanoma adhesion and migration under flow conditions.International journal of cancer · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The objective of this paper is to introduce some novel in vitro applications in characterizing human melanoma cell protrusion and migration in response to soluble extracellular matrix protein stimulation. Specifically, we describe two assay systems: (1) dual-micropipette manipulation and (2) flow-migration chamber. Applications of the dual-micropipet technique provided kinetic measure of cell movement, cyclic pseudopod protrusion, and subsequent cell locomotion governed by chemotactic molecular transport dynamics. Chemotactic concentration gradient was found to influence significantly pseudopod protrusion frequency and locomotion speed, but not the protrusion extension. To further characterize active tumor cell extravasation, a process that involves dynamic tumor cell adhesion to vascular endothelium under flow conditions and subsequent transendothelial migration in response to chemotactic signals from the interstitial space, we developed a flow-migration chemotaxis system. This assay enabled characterization of tumor cell transcellular migration in terms of chemotactic signal gradients, shear forces, and cell-substrate adhesion. Results suggest that shear flow plays significant roles in tumor cell extravasation that is regulated by both tumor cell motility and tumor cell adhesion to endothelial molecules in a cooperative process.
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