ArticleJournal of virology2002
Construction and manipulation of an infectious clone of the bovine herpesvirus 1 genome maintained as a bacterial artificial chromosome.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Genomic assembly, rescue, and characterization of a functional pseudorabies virus.Virologica Sinica · 2026Article
- Review
- Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antiviralsFrontiers in veterinary science · 2024Article
- Isolation and Identification of a Genotype C Bovine Parainfluenza Virus Type 3 and Its Pathogenicity in Albino Guinea Pigs.Transboundary and emerging diseases · 2023Article
- Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus.Frontiers in microbiology · 2016Article
- Construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain.Virology journal · 2013Article
- Phylogenetic analysis of the bovine parainfluenza virus type 3 from cattle herds revealing the existence of a genotype A strain in China.Virus genes · 2012Article
- Review
- Viral bacterial artificial chromosomes: generation, mutagenesis, and removal of mini-F sequences.Journal of biomedicine & biotechnology · 2012Review
- Recent advances in cloning herpesviral genomes as infectious bacterial artificial chromosomes.Cell cycle (Georgetown, Tex.) · 2011Review
- Herpesvirus BACs: past, present, and future.Journal of biomedicine & biotechnology · 2011Review
- Cloning of Bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes.BMC research notes · 2009Article
- Repertoire of bovine miRNA and miRNA-like small regulatory RNAs expressed upon viral infection.PloS one · 2009Article
- A bovine herpesvirus type 1 mutant virus specifying a carboxyl-terminal truncation of glycoprotein E is defective in anterograde neuronal transport in rabbits and calves.Journal of virology · 2008Article
- An overlapping bacterial artificial chromosome system that generates vectorless progeny for channel catfish herpesvirus.Journal of virology · 2008Article
- Production of high-titer Epstein-Barr virus recombinants derived from Akata cells by using a bacterial artificial chromosome system.Journal of virology · 2004Article
- Cloning of the full-length rhesus cytomegalovirus genome as an infectious and self-excisable bacterial artificial chromosome for analysis of viral pathogenesis.Journal of virology · 2003Article
- DNA modification and functional delivery into human cells using Escherichia coli DH10B.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably propagated over multiple generations of its host. BAC DNA recovered from DH10B cells and transfected into bovine cells produced a cytopathic effect which was indistinguishable from that of the parent virus. Analysis of the replication kinetics of the viral progeny indicated that insertion of the BAC vector into the thymidine kinase gene did not affect viral replication. Specific manipulation of the BAC was demonstrated by deleting the gene encoding glycoprotein E by homologous recombination in DH10B cells facilitated by GET recombination. These studies illustrate that the propagation and manipulation of herpesviruses in bacterial systems will allow for rapid and accurate characterization of BoHV-1 genes. In turn, this will allow for the full utilization of BoHV-1 as a vaccine vector.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.