ArticleGenome biology2002
Microarray profile of differentially expressed genes in a monkey model of allergic asthma.
Article in Genome biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 100 citations in OpenAlex.
- Allergic asthma biomarkers using systems approaches.Frontiers in genetics · 2014Review
- Segmental allergen challenge enhances chitinase activity and levels of CCL18 in mild atopic asthma.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2013Article
- How can microarrays unlock asthma?Journal of allergy · 2012Article
- Lung gene expression in a rhesus allergic asthma model correlates with physiologic parameters of disease and exhibits common and distinct pathways with human asthma and a mouse asthma model.The American journal of pathology · 2011Article
- Allergic asthma: influence of genetic and environmental factors.The Journal of biological chemistry · 2011Review
- Phenocopy--a strategy to qualify chemical compounds during hit-to-lead and/or lead optimization.PloS one · 2010Article
- Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis.PloS one · 2010Article
- Role of breast regression protein-39/YKL-40 in asthma and allergic responses.Allergy, asthma & immunology research · 2010Article
- Dexamethasone and FK506 inhibit expression of distinct subsets of chemokines in human mast cells.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Disease-specific gene expression profiling in multiple models of lung disease.American journal of respiratory and critical care medicine · 2008Article
- Complex regulation of pulmonary inflammation and fibrosis by CCL18.The American journal of pathology · 2007Article
- High-density rhesus macaque oligonucleotide microarray design using early-stage rhesus genome sequence information and human genome annotations.BMC genomics · 2007Article
- Article
- PKCalpha mediates CCL18-stimulated collagen production in pulmonary fibroblasts.American journal of respiratory cell and molecular biology · 2006Article
- Understanding genomics: implications for the emergency medicine physician and the treatment of asthma.Pediatric emergency care · 2006Review
- Microarray profiling of lymphocytes in internal diseases with an altered immune response: potential and methodology.Mediators of inflammation · 2005Review
- Involvement of CC chemokine ligand 18 (CCL18) in normal and pathological processes.Journal of leukocyte biology · 2005Review
- Chemokine networks in atopic dermatitis: traffic signals of disease.Current allergy and asthma reports · 2005Review
- Nonhuman primate models of asthma.The Journal of experimental medicine · 2005Article
- Gene expression profile in response to Xanthomonas axonopodis pv. manihotis infection in cassava using a cDNA microarray.Plant molecular biology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInhalation of Ascaris suum antigen by allergic monkeys causes an immediate bronchoconstriction and delayed allergic reaction, including a pulmonary inflammatory infiltrate. To identify genes involved in this process, the gene-expression pattern of allergic monkey lungs was profiled by microarrays. Monkeys were challenged by inhalation of A. suum antigen or given interleukin-4 (IL-4) treatment; lung tissue was collected at 4, 18 or 24 h after antigen challenge or 24 h after IL-4. Each challenged monkey lung was compared to a pool of normal, unchallenged monkey lungs.
resultsOf the approximately 40,000 cDNAs represented on the microarray, expression levels of 169 changed by more than 2.5-fold in at least one of the pairwise probe comparisons; these cDNAs encoded 149 genes, of which two thirds are known genes. The largest number of regulated genes was observed 4 h after challenge. Confirmation of differential expression in the original tissue was obtained for 95% of a set of these genes using real-time PCR. Cluster analysis revealed at least five groups of genes with unique expression patterns. One cluster contained genes for several chemokine mediators including eotaxin, PARC, MCP-1 and MCP-3. Genes involved in tissue remodeling and antioxidant responses were also identified as regulated by antigen and IL-4 or by antigen only.
conclusionThis study provides a large-scale profile of gene expression in the primate lung following allergen or IL-4 challenge. It shows that microarrays, with real-time PCR, are a powerful tool for identifying and validating differentially expressed genes in a disease model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.