ArticleThe Biochemical journal2002
TcGPXII, a glutathione-dependent Trypanosoma cruzi peroxidase with substrate specificity restricted to fatty acid and phospholipid hydroperoxides, is localized to the endoplasmic reticulum.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 111 citations in OpenAlex.
- Mitochondrial CaAntioxidants & redox signaling · 2022Review
- Antioxidant defence system as a rational target for Chagas disease and Leishmaniasis chemotherapy.Memorias do Instituto Oswaldo Cruz · 2022Review
- Article
- The mitochondrial peroxiredoxin displays distinct roles in different developmental stages of African trypanosomes.Redox biology · 2020Article
- Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis inFrontiers in cellular and infection microbiology · 2019Review
- Kinetics, subcellular localization, and contribution to parasite virulence of aProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Ultrastructural and physiological changes induced by different stress conditions on the human parasite Trypanosoma cruzi.Cell stress & chaperones · 2017Article
- Trypanosoma cruzi Needs a Signal Provided by Reactive Oxygen Species to Infect Macrophages.PLoS neglected tropical diseases · 2016Article
- Killer lymphocytes use granulysin, perforin and granzymes to kill intracellular parasites.Nature medicine · 2016Article
- A ComparativeEvolutionary bioinformatics online · 2016Article
- The Trypanosoma cruzi vitamin C dependent peroxidase confers protection against oxidative stress but is not a determinant of virulence.PLoS neglected tropical diseases · 2015Article
- Evasion of the Immune Response by Trypanosoma cruzi during Acute Infection.Frontiers in immunology · 2015Review
- Trypanosoma cruzi antioxidant enzymes as virulence factors in Chagas disease.Antioxidants & redox signaling · 2013Review
- Enzymes of the antioxidant network as novel determiners of Trypanosoma cruzi virulence.International journal for parasitology · 2009Article
- Trypanosoma cruzi: antiproliferative effect of indole phytoalexins on intracellular amastigotes in vitro.Experimental parasitology · 2009Article
- Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite.The Biochemical journal · 2008Article
- The terminal step in vitamin C biosynthesis in Trypanosoma cruzi is mediated by a FMN-dependent galactonolactone oxidase.The Biochemical journal · 2007Article
- A comparative study of type I and type II tryparedoxin peroxidases in Leishmania major.The FEBS journal · 2007Article
- The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements.International journal for parasitology · 2007Article
- Vitamin C biosynthesis in trypanosomes: a role for the glycosome.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Until recently, it had been thought that trypanosomes lack glutathione peroxidase activity. Here we report the subcellular localization and biochemical properties of a second glutathione-dependent peroxidase from Trypanosoma cruzi (TcGPXII). TcGPXII is a single-copy gene which encodes a 16 kDa protein that appears to be specifically dependent on glutathione as the source of reducing equivalents. Recombinant TcGPXII was purified and shown to have peroxidase activity towards a narrow substrate range, restricted to hydroperoxides of fatty acids and phospholipids. Analysis of the pathway revealed that TcGPXII activity could be readily saturated by glutathione and that the peroxidase functioned by a Ping Pong mechanism. Enzyme reduction was shown to be the rate-limiting step in this pathway. Using immunofluorescence, TcGPXII was shown to co-localize with a homologue of immunoglobulin heavy-chain binding protein (BiP), a protein restricted to the endoplasmic reticulum and Golgi. As the smooth endoplasmic reticulum is the site of phospholipid and fatty acid biosynthesis, this suggests that TcGPXII may play a specific role in the T. cruzi oxidative defence system by protecting newly synthesized lipids from peroxidation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.