ArticleThe Biochemical journal2002
Expression of solute carrier 7A4 (SLC7A4) in the plasma membrane is not sufficient to mediate amino acid transport activity.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 71 citations in OpenAlex.
- Structural basis for pH-responsive amino acid transport via SLC7A4.Nature communications · 2026Article
- Research progress on the role of cationic amino acid transporter (CAT) family members in malignant tumors and immune microenvironment.Amino acids · 2023Review
- The Identification of the Metabolism Subtypes of Skin Cutaneous Melanoma Associated With the Tumor Microenvironment and the Immunotherapy.Frontiers in cell and developmental biology · 2021Article
- Integrative Analysis of Methylation and Copy Number Variations of Prostate Adenocarcinoma Based on Weighted Gene Co-expression Network Analysis.Frontiers in oncology · 2021Article
- Membrane Transporters and Channels in Melanoma.Reviews of physiology, biochemistry and pharmacology · 2021Review
- In the line-up: deleted genes associated with DiGeorge/22q11.2 deletion syndrome: are they all suspects?Journal of neurodevelopmental disorders · 2019Review
- Uptake and metabolism of arginine impact Plasmodium development in the liver.Scientific reports · 2017Article
- Real-time functional characterization of cationic amino acid transporters using a new FRET sensor.Pflugers Archiv : European journal of physiology · 2016Article
- Cardiovascular Action of Insulin in Health and Disease: Endothelial L-Arginine Transport and Cardiac Voltage-Dependent Potassium Channels.Frontiers in physiology · 2016Review
- Mouse and human CRKL is dosage sensitive for cardiac outflow tract formation.American journal of human genetics · 2015Article
- L-arginine stimulates CAT-1-mediated arginine uptake and regulation of inducible nitric oxide synthase for the growth of chick intestinal epithelial cells.Molecular and cellular biochemistry · 2015Article
- Membrane transporters in a human genome-scale metabolic knowledgebase and their implications for disease.Frontiers in physiology · 2014Review
- Placental accommodations for transport and metabolism during intra-uterine crowding in pigs.Journal of animal science and biotechnology · 2014Review
- The Concise Guide to PHARMACOLOGY 2013/14: transporters.British journal of pharmacology · 2013Article
- A chimera carrying the functional domain of the orphan protein SLC7A14 in the backbone of SLC7A2 mediates trans-stimulated arginine transport.The Journal of biological chemistry · 2012Article
- Molecular organization of Drosophila neuroendocrine cells by Dimmed.Current biology : CB · 2011Article
- Expression and analysis of two novel rat organic cation transporter homologs, SLC22A17 and SLC22A23.Molecular and cellular biochemistry · 2011Article
- Mutation of a TADR protein leads to rhodopsin and Gq-dependent retinal degeneration in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Characterization of a membrane protein (VP001L) from infectious spleen and kidney necrosis virus (ISKNV).Virus genes · 2008Article
- Structure and function of cationic amino acid transporters (CATs).The Journal of membrane biology · 2006Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Member 4 of human solute carrier family 7 (SLC7A4) exhibits significant sequence homology with the SLC7 subfamily of human cationic amino acid transporters (hCATs) [Sperandeo, Borsani, Incerti, Zollo, Rossi, Zuffardi, Castaldo, Taglialatela, Andria and Sebastio (1998) Genomics 49, 230-236]. It is therefore often referred to as hCAT-4 even though no convincing transport activity has been shown for this protein. We expressed SLC7A4 in Xenopus laevis oocytes, but could not detect any transport activity for cationic, neutral or anionic amino acids or for the polyamine putrescine. In addition, human glioblastoma cells stably overexpressing a fusion protein between SLC7A4 and the enhanced green fluorescent protein (EGFP) did not exhibit an increased transport activity for l-arginine. The lack of transport activity was not due to a lack of SLC7A4 protein expression in the plasma membrane, as in both cell types SLC7A4-EGFP exhibited a similar subcellular localization and level of protein expression as functional hCAT-EGFP proteins. The expression of SLC7A4 can be induced in NT2 teratocarcinoma cells by treatment with retinoic acid. However, also for this endogenously expressed SLC7A4, we could not detect any transport activity for l-arginine. Our data demonstrate that the expression of SLC7A4 in the plasma membrane is not sufficient to induce an amino acid transport activity in X. laevis oocytes or human cells. Therefore, SLC7A4 is either not an amino acid transporter or it needs additional (protein) factor(s) to be functional.
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