ArticleThe Biochemical journal2002
Specific detection of the endogenous transient receptor potential (TRP)-1 protein in liver and airway smooth muscle cells using immunoprecipitation and Western-blot analysis.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Emodin Interferes With Nitroglycerin-Induced Migraine in Rats Through CGMP-PKG Pathway.Frontiers in pharmacology · 2021Article
- Article
- Review
- TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle.Biochimica et biophysica acta · 2013Article
- TRP channel gene expression in the mouse retina.Vision research · 2011Article
- Molecular expression and functional role of canonical transient receptor potential channels in airway smooth muscle cells.Advances in experimental medicine and biology · 2011Review
- Functional role of canonical transient receptor potential 1 and canonical transient receptor potential 3 in normal and asthmatic airway smooth muscle cells.American journal of respiratory cell and molecular biology · 2010Article
- Differential expression of canonical (classical) transient receptor potential channels in guinea pig enteric nervous system.The Journal of comparative neurology · 2008Article
- Store-operated Ca(2+) entry in platelets occurs independently of transient receptor potential (TRP) C1.Pflugers Archiv : European journal of physiology · 2008Article
- Calcium influx mechanisms underlying calcium oscillations in rat hepatocytes.Hepatology (Baltimore, Md.) · 2008Article
- Interaction with dopamine D2 receptor enhances expression of transient receptor potential channel 1 at the cell surface.Biochimica et biophysica acta · 2008Article
- Role of transient receptor potential C3 in TNF-alpha-enhanced calcium influx in human airway myocytes.American journal of respiratory cell and molecular biology · 2006Article
- Specific detection and semi-quantitative analysis of TRPC4 protein expression by antibodies.Pflugers Archiv : European journal of physiology · 2005Review
- Functional role of TRPC proteins in native systems: implications from knockout and knock-down studies.The Journal of physiology · 2005Review
- Non-selective cationic channels of smooth muscle and the mammalian homologues of Drosophila TRP.The Journal of physiology · 2004Review
- Chronic lithium treatment of B lymphoblasts from bipolar disorder patients reduces transient receptor potential channel 3 levels.The pharmacogenomics journal · 2004Article
- Evidence that TRPC1 (transient receptor potential canonical 1) forms a Ca(2+)-permeable channel linked to the regulation of cell volume in liver cells obtained using small interfering RNA targeted against TRPC1.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although there are numerous reports of the presence of mRNA encoding the transient receptor potential (TRP)-1 protein in animal cells and of the detection of the heterologously expressed TRP-1 protein by Western-blot analysis, it has proved difficult to unequivocally detect endogenous TRP-1 proteins. A combination of immunoprecipitation and Western-blot techniques, employing a polyclonal antibody and a monoclonal antibody respectively, was developed. Using this technique, a band of approx. 80 kDa was detected in extracts of H4-IIE rat liver hepatoma cell line and guinea-pig airway smooth muscle (ASM) cells transfected with human TRPC-1 cDNA. In extracts of untransfected H4-IIE cells, ASM cells, rat brain and guinea-pig brain, a band of approx. 92 kDa was detected. Reverse transcriptase PCR experiments detected cDNA encoding both the alpha- and beta-isoforms of TRP-1 in H4-IIE cells. Treatment of protein extracts with peptide N-glycosidase F indicated that the 92 kDa band represents an N-glycosylated protein. Western blots conducted with a commercial polyclonal anti-(TRP-1) antibody (Alm) detected a band of 120 kDa in extracts of H4-IIE cells and guinea-pig ASM cells. A combination of immunoprecipitation and Western-blotting techniques with the Alm antibody did not detect any bands at 92 kDa or 120 kDa in extracts of H4-IIE and ASM cells. It is concluded that (a) the 92-kDa band detected in untransfected H4-IIE and ASM cells corresponds to the N-glycosylated beta-isoform of endogenous TRP-1, (b) the combined immunoprecipitation and Western-blot approach, employing two different antibodies, provides a reliable and specific procedure for detecting endogenous TRP-1 proteins, and (c) that caution is required in developing and utilizing anti-(TRP-1) antibodies.
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