ArticleWorld journal of gastroenterology2002
Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 12 citations in OpenAlex.
- Production of factor VIII by human liver sinusoidal endothelial cells transplanted in immunodeficient uPA mice.PloS one · 2013Trial
- Pivotal role of TLR4 receptors in alcohol-induced neuroinflammation and brain damage.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor and its intervention by interleukin-10 in experimental hepatic fibrosis.World journal of gastroenterology · 2003Article
- Role of NF-kB in multiple organ dysfunction during acute obstructive cholangitis.World journal of gastroenterology · 2003Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo observe expression of CD14 protein and CD14 gene in rat liver sinusoidal endothelial cells (LSECs) during endotoxemia, and the role of CD14 protein in the activation of lipopolysaccharide (LPS)-induced LSECs.
methodsWistar rat endotoxemia model was established first by injection of a dose of LPS (5mg/kg, Escherichia coli O111:B4 ) via the tail vein, then sacrificed after 0 h,3h,6h, 12h, and 24h, respectively. LSECs were isolated from normal and LPS-injected rats by an in situ collagenase perfusion technique. The isolated LSECs were incubated with rabbit anti-rat CD14 polyclonal antibody, then stained with goat anti rabbit IgG conjugated fluorescein isothiocyanate(FITC) and flow cytometric analysis (FCM) was performed. The percentage and mean fluorescence intensity (MFI) of CD14-positive cells were taken as the indexes. LSECs were collected to measure the expression of CD14 mRNA by in situ hybridization analysis. The isolated LSECs from normal rats were incubated firstly with anti-CD14 antibody, then stimulated with different concentrations of LPS, and the supernatants of these cells were then collected for measuring the levels of tumor necrosis factor (TNF)-a and Interleukin (IL)-6 with ELISA.
resultsIn rats with endotoxemia, LSECs displayed a strong MFI distinct from that of control rats. CD14 positive cells in rats with endotoxemia were 54.32%, 65.83%, 85.64%, and 45.65% at 3h, 6h, 12h, and 24h respectively, there was significant difference when compared to normal group of animals (4.45%)(P<0.01). The expression of CD14 mRNA in isolated LSECs was stronger than that in control rats. In LPS group, the levels of TNF-alpha and IL-6 were 54+/-6 ng.L(-1), 85+/-9 ng.L(-1), 206+/-22 ng.L(-1), 350+/-41 ng.L(-1), 366+/-42 ng.L(-1) and 103+/-11 ng.L(-1), 187+/-20 ng.L(-1), 244+/-26 ng.L(-1), 290+/-31 ng.L(-1), and 299+/-34 ng.L(-1), respectively at different concentration points. In anti-CD14 group, the levels of TNF-alpha and IL-6 were 56+/-5 ng.L(-1), 67+/-8 ng.L(-1), 85+/-10 ng.L(-1), 113+/-12 ng.L(-1), 199+/-22 ng.L(-1) and 104+/-12 ng.L(-1), 125+/-12 ng.L(-1), 165+/-19 ng.L(-1), 185+/-21 ng.L(-1), and 222+/-23 ng.L(-1), respectively at different concentration points. There was significant difference between the two groups (P<0.01).
conclusionLSECs can synthesize CD14 protein and express CD14 gene during endotoxemia. CD14 protein plays an important role in the activation of LPS-induced LSECs. This finding has important implications for the understanding of the mechanisms by which LPS may injure liver sinusoidal endothelial cells during sepsis.
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