ArticleWorld journal of gastroenterology2002
Induction of apoptosis by TPA and VP-16 is through translocation of TR3.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Therapeutic mechanism of ginkgo biloba exocarp polysaccharides on gastric cancer.World journal of gastroenterology · 2003Trial
- The Paradoxical Roles of Orphan Nuclear Receptor 4A (NR4A) in Cancer.Molecular cancer research : MCR · 2021Review
- Targeting truncated RXRα for cancer therapy.Acta biochimica et biophysica Sinica · 2016Review
- The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-Cells from Endoplasmic Reticulum (ER) Stress-mediated Apoptosis.The Journal of biological chemistry · 2015Article
- Prolyl isomerase Pin1 stabilizes and activates orphan nuclear receptor TR3 to promote mitogenesis.Oncogene · 2012Article
- Expression of Nur77 induced by an n-butylidenephthalide derivative promotes apoptosis and inhibits cell growth in oral squamous cell carcinoma.Investigational new drugs · 2012Article
- The expression of protein kinase B in gastric cancer cell apoptosis induced by 12-O-tetradecanoylphorbol-1, 3-acetate.Cellular & molecular biology letters · 2009Article
- Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues.Cancer research · 2008Article
- p53 and Nur77/TR3 - transcription factors that directly target mitochondria for cell death induction.Oncogene · 2006Review
- Article
- Development of resistance to the atypical retinoid, ST1926, in the lung carcinoma cell line H460 is associated with reduced formation of DNA strand breaks and a defective DNA damage response.Neoplasia (New York, N.Y.) · 2005Article
- Early events in the induction of apoptosis in ovarian carcinoma cells by CD437: activation of the p38 MAP kinase signal pathway.Oncogene · 2003Article
- Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells.World journal of gastroenterology · 2003Article
- Degradation of retinoid X receptor alpha by TPA through proteasome pathway in gastric cancer cells.World journal of gastroenterology · 2003Article
- Peroxisome proliferator-activated receptor gamma ligands inhibit cell growth and induce apoptosis in human liver cancer BEL-7402 cells.World journal of gastroenterology · 2003Article
- PPARgamma pathway activation results in apoptosis and COX-2 inhibition in HepG2 cells.World journal of gastroenterology · 2003Article
- Activation of JNK by TPA promotes apoptosis via PKC pathway in gastric cancer cells.World journal of gastroenterology · 2002Article
- Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide.World journal of gastroenterology · 2002Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the role of TR3 in induction of apoptosis in gastric cancer cells.
methodsHuman gastric cancer cell line, MGC80-3, was used. Expression of TR3 mRNA and its protein was detected by Northern blot and Western blot. Localization of TR3 protein was showed by immunofluorescence analysis under laser-scanning confocal microscope. Apoptotic morphology was observed by DAPI fluorescence staining, and apoptotic index was counted among 1000 cells randomly. Stable transfection assay was carried out by Lipofectamine.
resultsTreatment of MGC80-3 cells with TPA and VP-16 resulted in apoptosis, accompanied by the repression of Bcl-2 protein in a time-dependent manner. At the same time, TPA and VP-16 also up-regulated expression level of TR3 mRNA in MGC80-3 cells that expressed TR3 mRNA. When antisense-TR3 expression vector was transfected into the cells, expression of TR3 protein was repressed. In this case, TPA and VP-16 did not induce apoptosis. In addition, TPA and VP-16-induced apoptosis involved in translocation of TR3. In MGC80-3 cells, TR3 localized concentrative in nucleus, after treatment of cells with TPA and VP-16, TR3 translocated from nucleus to cytosol obviously. However, when this nuclear translocation was blocked by LMB, apoptosis was not occurred in MGC80-3 cells even in the presence of TPA and VP-16.
conclusionInduction of apoptosis by TPA and VP-16 is through induction of TR3 expression and translocation of TR3 from nucleus to cytosol, which may be a novel signal pathway for TR3, and represent the new biological function of TR3 to exert its effect on apoptosis in gastric cancer cells.
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