ArticleThe Journal of physiology2002
Activation and sensitisation of low and high threshold afferent fibres mediated by P2X receptors in the mouse urinary bladder.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 206 citations in OpenAlex.
- Early urodynamic profiles predict lower urinary tract recovery after spinal cord injury in female rats.Physiological reports · 2026Article
- Functional characterization and classification of mechanosensitive bladder afferents.bioRxiv : the preprint server for biology · 2026Article
- Article
- Review
- Intravesical Injection of Abobotulinumtoxin-A in Patients with Bladder Pain Syndrome/Interstitial Cystitis.Urology research & practice · 2023Article
- Sensory Neurons, PIEZO Channels and PAC1 Receptors Regulate the Mechanosensitive Release of Soluble Ectonucleotidases in the Murine Urinary Bladder Lamina Propria.International journal of molecular sciences · 2023Article
- Afferent nerve activity in a mouse model increases with faster bladder filling rates in vitro, but voiding behavior remains unaltered in vivo.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Review
- Activation of uroepithelial 5-HTFrontiers in physiology · 2022Article
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity.Scientific reports · 2021Article
- Activation of MrgprA3 and MrgprC11 on Bladder-Innervating Afferents Induces Peripheral and Central Hypersensitivity to Bladder Distension.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Transient receptor potential channels in sensory mechanisms of the lower urinary tract.Nature reviews. Urology · 2021Review
- Emerging roles of the TRPV4 channel in bladder physiology and dysfunction.The Journal of physiology · 2021Review
- Bladder urothelium converts bacterial lipopolysaccharide information into neural signaling via an ATP-mediated pathway to enhance the micturition reflex for rapid defense.Scientific reports · 2020Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- The Role of Purinergic Receptors in the Circadian System.International journal of molecular sciences · 2020Review
- Review
- Choosing to urinate. Circuits and mechanisms underlying voluntary urination.Current opinion in neurobiology · 2020Review
- Clinical application of intravesical botulinum toxin type A for overactive bladder and interstitial cystitis.Investigative and clinical urology · 2020Review
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been proposed that extracellular ATP may be involved in visceral mechanosensory transduction by activating ligand-gated ion channels (P2X receptors). In this study, we have investigated the effects of the P2X(3) agonist alpha,beta-methylene ATP (alpha,beta-meATP) and antagonist 2',3'-O-trinitrophenyl-ATP (TNP-ATP) on pelvic afferents innervating the urinary bladder using an in vitro mouse bladder-pelvic nerve preparation. Intravesical application of alpha,beta-meATP (0.03-1 mM) increased multifibre discharges in a concentration-dependent manner. The agonist potentiated, whereas TNP-ATP (0.03 mM) attenuated, the multifibre responses to bladder distensions. Single-unit analysis revealed that both high threshold (HT) fibres (> 15 mmHg; known to be associated with nociception) and low threshold (LT) fibres (< 15 mmHg; probably associated with non-nociceptive events) could be induced to discharge by intravesical alpha,beta-meATP (1 mM, 0.1 ml). The response of the vast majority (21/22, 95.5 %) of HT fibres to bladder distensions was enhanced with a significantly reduced threshold and an increased peak response after exposure to the agonist. On the other hand, 59.7 % (46/77) of LT fibres showed a greater peak and a slightly reduced threshold for response to bladder distension in the presence of alpha,beta-meATP. An additional 11 'silent' fibres became mechanosensitive after exposure to alpha,beta-meATP. TNP-ATP (0.03 mM) did not affect the threshold of LT fibres, but it reduced the peak response of some (22/51, 43.1 %) LT fibres. Conversely, the antagonist resulted in a markedly elevated threshold and reduced peak activity in the majority (13/16, 81.3 %) of HT fibres. The results support the view that P2X(3) receptor-mediated mechanisms contribute to both nociceptive and non-nociceptive (physiological) mechanosensory transduction in the urinary bladder.
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