ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Regional calcium regulation within cultured Drosophila neurons: effects of altered cAMP metabolism by the learning mutations dunce and rutabaga.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Dunce Phosphodiesterase Acts as a Checkpoint for Drosophila Long-Term Memory in a Pair of Serotonergic Neurons.Neuron · 2018Article
- Cyclic nucleotide signaling is required during synaptic refinement at the Drosophila neuromuscular junction.Developmental neurobiology · 2017Article
- Sleep restores behavioral plasticity to Drosophila mutants.Current biology : CB · 2015Article
- The role of cAMP in synaptic homeostasis in response to environmental temperature challenges and hyperexcitability mutations.Frontiers in cellular neuroscience · 2015Article
- Article
- Cyclic adenosine monophosphate metabolism in synaptic growth, strength, and precision: neural and behavioral phenotype-specific counterbalancing effects between dnc phosphodiesterase and rut adenylyl cyclase mutations.Journal of neurogenetics · 2012Article
- State-dependent disruption of short-term facilitation due to overexpression of the apPDE4 supershort form in Aplysia.Molecules and cells · 2011Article
- Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor.Developmental neurobiology · 2008Article
- Temperature-dependent developmental plasticity of Drosophila neurons: cell-autonomous roles of membrane excitability, Ca2+ influx, and cAMP signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Drosophila as a genetic and cellular model for studies on axonal growth.Neural development · 2007Review
- Drosophila cacophony channels: a major mediator of neuronal Ca2+ currents and a trigger for K+ channel homeostatic regulation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dunce (dnc) and rutabaga (rut) mutations of Drosophila affect a cAMP-dependent phosphodiesterase and a Ca(2+)/CaM-regulated adenylyl cyclase, respectively. These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. The cellular phenotypes either are Ca(2+) dependent (neurotransmission and motility) or mediate a Ca(2+) rise (action potential generation). However, interrelations among these defects have not been addressed. We have established conditions for fura-2 imaging of Ca(2+) dynamics in the "giant" neuron culture system of Drosophila. Using high K(+) depolarization of isolated neurons, we observed a larger, faster, and more dynamic response from the growth cone than the cell body. This Ca(2+) increase depended on an influx through Ca(2+) channels and was suppressed by the Na(+) channel blocker TTX. Altered cAMP metabolism by the dnc and rut mutations reduced response amplitude in the growth cone while prolonging the response within the soma. The enhanced spatial resolution of these larger cells allowed us to analyze Ca(2+) regulation within distinct domains of mutant growth cones. Modulation by a previous conditioning stimulus was altered in terms of response amplitude and waveform complexity. Furthermore, rut disrupted the distinction in Ca(2+) responses observed between the periphery and central domain of growth cones with motile filopodia.
Indexed as
Identifiers
12040051PMC6758795W2129620979What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.