ArticleApplied and environmental microbiology2002
Rapid detection and enumeration of Naegleria fowleri in surface waters by solid-phase cytometry.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Article
- Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization ofAnnals of medicine and surgery (2012) · 2024Review
- Growth dynamic of biofilm-associatedFrontiers in microbiology · 2024Article
- An Optimized Most Probable Number (MPN) Method to Assess the Number of Thermophilic Free-Living Amoebae (FLA) in Water Samples.Pathogens (Basel, Switzerland) · 2020Article
- Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay.Parasitology research · 2013Article
- Total and viable Legionella pneumophila cells in hot and natural waters as measured by immunofluorescence-based assays and solid-phase cytometry.Applied and environmental microbiology · 2011Article
- Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry.Nature protocols · 2009Article
- Survey for the presence of specific free-living amoebae in cooling waters from Belgian power plants.Parasitology research · 2007Article
- Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new method for the rapid and accurate detection of pathogenic Naegleria fowleri amoebae in surface environmental water was developed. The method is based on an immunofluorescent assay combined with detection by solid-phase cytometry. In this study we developed and compared two protocols using different reporter systems conjugated to antibodies. The monoclonal antibody Ac5D12 was conjugated with biotin and horseradish peroxidase, and the presence of cells was revealed with streptavidin conjugated to both R-phycoerythrin and cyanine Cy5 (RPE-Cy5) and tyramide-fluorescein isothiocyanate, respectively. The RPE-Cy5 protocol was the most efficient protocol and allowed the detection of both trophozoite and cyst forms in water. The direct counts obtained by this new method were not significantly different from those obtained by the traditional culture approach, and results were provided within 3 h. The sensitivity of the quantitative method is 200 cells per liter. The limit is due only to the filtration capacity of the membrane used.
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Registered trials
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