ArticleApplied and environmental microbiology2002
Bacteriophage resistance of a deltathyA mutant of Lactococcus lactis blocked in DNA replication.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 25 citations in OpenAlex.
- Ribosome profiling reveals downregulation of UMP biosynthesis as the major early response to phage infection.Microbiology spectrum · 2024Article
- Increased mutations in lipopolysaccharide biosynthetic genes cause time-dependent development of phage resistance inAntimicrobial agents and chemotherapy · 2024Article
- The art of strain improvement of industrial lactic acid bacteria without the use of recombinant DNA technology.Microbial cell factories · 2014Review
- The MG1363 and IL1403 laboratory strains of Lactococcus lactis and several dairy strains are diploid.Journal of bacteriology · 2010Article
- Detection of bacteriophage-infected cells of Lactococcus lactis by using flow cytometry.Applied and environmental microbiology · 2007Article
- High-level acetaldehyde production in Lactococcus lactis by metabolic engineering.Applied and environmental microbiology · 2005Article
- Increasing acidification of nonreplicating Lactococcus lactis deltathyA mutants by incorporating ATPase activity.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The thyA gene, which encodes thymidylate synthase (TS), of Lactococcus lactis CHCC373 was sequenced, including the upstream and downstream regions. We then deleted part of thyA by gene replacement. The resulting strain, MBP71 deltathyA, was devoid of TS activity, and in media without thymidine, such as milk, there was no detectable dTTP pool in the cells. Hence, DNA replication was abolished, and acidification by MBP71 was completely unaffected by the presence of nine different phages tested at a multiplicity of infection (MOI) of 0.1. Nonreplicating MBP71 must be inoculated at a higher level than CHCC373 to achieve a certain pH within a specified time. For a pH of 5.2 to be reached in 6 h, the inoculation level of MBP71 must be 17-fold higher than for CHCC373. However, by adding a limiting amount of thymidine this could be lowered to just 5-fold the normal amount, while acidification was unaffected with MBP71 up to an MOI of 0.01. It was found that nonreplicating MBP71 produced largely the same products as CHCC373, though the acetaldehyde production of the former was higher.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.