ArticleApplied and environmental microbiology2002
Identification and isolation of genes involved in poly(beta-hydroxybutyrate) biosynthesis in Azospirillum brasilense and characterization of a phbC mutant.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 70 citations in OpenAlex.
- Bioinformatic and functional analysis of a PHB polymerase (PhbC) from Azospirillum baldaniorum.Journal, genetic engineering & biotechnology · 2024Article
- Review
- Pleiotropic Effects of PhaR Regulator inInternational journal of molecular sciences · 2024Article
- Polyhydroxybutyrate Metabolism inPolymers · 2023Review
- Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer fromPolymers · 2022Article
- Genetic Circuit Design in Rhizobacteria.Biodesign research · 2022Review
- Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces.mSystems · 2021Article
- Redox Regulation in Diazotrophic Bacteria in Interaction with Plants.Antioxidants (Basel, Switzerland) · 2021Review
- Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in Azospirillum brasilense Sp7.AMB Express · 2019Article
- A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Metabolic adaptations of Azospirillum brasilense to oxygen stress by cell-to-cell clumping and flocculation.Applied and environmental microbiology · 2015Article
- Transcriptome analysis of cyst formation in Rhodospirillum centenum reveals large global changes in expression during cyst development.BMC genomics · 2015Article
- Cellular responses during morphological transformation in Azospirillum brasilense and Its flcA knockout mutant.PloS one · 2014Article
- Phylogenetic analysis of eukaryotic thiolases suggests multiple proteobacterial origins.Journal of molecular evolution · 2005Article
- Engineering the nifH promoter region and abolishing poly-beta-hydroxybutyrate accumulation in Rhizobium etli enhance nitrogen fixation in symbiosis with Phaseolus vulgaris.Applied and environmental microbiology · 2004Article
- Involvement of the reserve material poly-beta-hydroxybutyrate in Azospirillum brasilense stress endurance and root colonization.Applied and environmental microbiology · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Like many other prokaryotes, rhizobacteria of the genus Azospirillum produce high levels of poly(beta-hydroxybutyrate) (PHB) under suboptimal growth conditions. Utilization of PHB by bacteria under stress has been proposed as a mechanism that favors their compatible establishment in competitive environments, thus showing great potential for the improvement of bacterial inoculants for plants and soils. The three genes that are considered to be essential in the PHB biosynthetic pathway, phbA (beta-ketothiolase), phbB (acetoacetyl coenzyme A reductase), and phbC (PHB synthase), were identified in Azospirillum brasilense strain Sp7, cloned, and sequenced. The phbA, -B, and -C genes were found to be linked together and located on the chromosome. An A. brasilense phbC mutant was obtained by insertion of a kanamycin resistance cassette within the phbC gene. No PHB production was detected in this mutant. The capability of the wild-type strain to endure starvation conditions was higher than that of the mutant strain. However, motility, cell aggregation, root adhesion, and exopolysaccharide (EPS) and capsular polysaccharide (CPS) production were higher in the phbC mutant strain than in the wild type.
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