ArticleApplied and environmental microbiology2002
Development of a flow cytometric method to analyze subpopulations of bacteria in probiotic products and dairy starters.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 146 citations in OpenAlex.
- A portable device for probiotic counting and viability assessment based on microfluidic chip and image recognition.iMetaOmics · 2025Article
- A streamlined workflow for a fast and cost-effective count of tyndallized probiotics using flow cytometry.Frontiers in microbiology · 2024Article
- Article
- Detection of Glycolytically Active Lacticaseibacillus paracasei Strain Shirota by Flow Cytometry Targeting the Efflux Activity of Fluorescent Dye: a Potential Tool for Quality Assessment of Probiotic Cells in Milk Products.Applied and environmental microbiology · 2023Article
- An Integrated Analytical Approach for the Characterization of Probiotic Strains in Food Supplements.Nutrients · 2022Article
- Dynamics of physiological responses of potentially probiotic fruit-derived Limosilactobacillus fermentum in apple and orange juices during refrigeration storage and exposure to simulated gastrointestinal conditions.Archives of microbiology · 2021Article
- Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry-A Review.Foods (Basel, Switzerland) · 2021Review
- Flow Cytometry to Assess the Counts and Physiological State ofFoods (Basel, Switzerland) · 2019Article
- Among Live and Dead Bacteria, the Optimization of Sample Collection and Processing Remains Essential in Recovering Gut Microbiota Components.Frontiers in microbiology · 2019Article
- Rapid assessment of viable but non-culturable Bacillus coagulans MTCC 5856 in commercial formulations using Flow cytometry.PloS one · 2018Article
- Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Flow cytometric evaluation of physico-chemical impact on Gram-positive and Gram-negative bacteria.Frontiers in microbiology · 2015Article
- Fourier transform infra-red spectroscopy and flow cytometric assessment of the antibacterial mechanism of action of aqueous extract of garlic (Allium sativum) against selected probiotic Bifidobacterium strains.BMC complementary and alternative medicine · 2014Article
- Evolution of microbiological analytical methods for dairy industry needs.Frontiers in microbiology · 2014Review
- Article
- Patterns of survival and volatile metabolites of selected Lactobacillus strains during long-term incubation in milk.Journal of microbiology (Seoul, Korea) · 2010Article
- Flow cytometry applications in the food industry.Journal of industrial microbiology & biotechnology · 2009Review
- Application of flow cytometry to segregated kinetic modeling based on the physiological states of microorganisms.Applied and environmental microbiology · 2007Article
- Use of flow cytometry to follow the physiological states of microorganisms in cider fermentation processes.Applied and environmental microbiology · 2006Article
- Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays.Applied and environmental microbiology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow cytometry (FCM) is a rapid and sensitive technique that can determine cell numbers and measure various physiological characteristics of individual cells by using appropriate fluorescent probes. Previously, we developed an FCM assay with the viability probes carboxyfluorescein diacetate (cFDA) and TOTO-1 [1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methyl-2,3dihydro(benzo-1,3-oxazole)-2-methylidene]-1-(3'-trimethylammoniumpropyl)-pyridinium tetraiodide] for (stressed) lactic acid bacteria (C. J. Bunthof, K. Bloemen, P. Breeuwer, F. M. Rombouts, and T. Abee, Appl. Environ. Microbiol. 67:2326-2335, 2001). cFDA stains intact cells with enzymatic activity, and TOTO-1 stains membrane-permeabilized cells. Here we used this assay to study the viability of bacterial suspensions in milk, dairy fermentation starters, and probiotic products. To facilitate FCM analysis of bacteria in milk, a commercially available milk-clearing solution was used. The procedure was optimized to increase the signal-to-noise ratio. FCM enumerations were accurate down to a concentration of 10(5) cells ml(-1). The level of retrieval of Lactobacillus plantarum WCFS 1 suspended in milk was high, and viability was not affected by the procedure. The plate counts for cleared samples of untreated cell suspensions were nearly as high as the total FCM counts, and the correlation was strong (r > 0.99). In dairy fermentation starters and in probiotic products the FCM total cell counts were substantially higher than the numbers of CFU. Three functional populations could be distinguished: culturable cells, cells that are intact and metabolically active but not culturable, and permeabilized cells. The proportions of the populations differed in the products tested. This FCM method provides tools to assess the functionality of different populations in fermentation starters and probiotic products.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.