ArticleApplied and environmental microbiology2002
Dothistroma pini, a forest pathogen, contains homologs of aflatoxin biosynthetic pathway genes.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Fungal Effectoromics: A World in Constant Evolution.International journal of molecular sciences · 2022Review
- Extracellular Vesicles fromJournal of fungi (Basel, Switzerland) · 2021Article
- Scientific Opinion on the risk to plant health posed byEFSA journal. European Food Safety Authority · 2013Article
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- Gene duplication, modularity and adaptation in the evolution of the aflatoxin gene cluster.BMC evolutionary biology · 2007Article
- Biodiversity of Aspergillus species in some important agricultural products.Studies in mycology · 2007Article
- Biosynthesis of dothistromin.Mycopathologia · 2006Review
- Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implications.Mycopathologia · 2006Review
- A polyketide synthase gene required for biosynthesis of the aflatoxin-like toxin, dothistromin.Mycopathologia · 2006Article
- Evolution of the aflatoxin gene cluster.Mycotoxin research · 2006Article
- Molecular cloning and genetic analysis of a symbiosis-expressed gene cluster for lolitrem biosynthesis from a mutualistic endophyte of perennial ryegrass.Molecular genetics and genomics : MGG · 2005Article
- Phylogenomic analysis of type I polyketide synthase genes in pathogenic and saprobic ascomycetes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homologs of aflatoxin biosynthetic genes have been identified in the pine needle pathogen Dothistroma pini. D. pini produces dothistromin, a difuranoanthraquinone toxin with structural similarity to the aflatoxin precursor versicolorin B. Previous studies with purified dothistromin suggest a possible role for this toxin in pathogenicity. By using an aflatoxin gene as a hybridization probe, a genomic D. pini clone was identified that contained four dot genes with similarity to genes in aflatoxin and sterigmatocystin gene clusters with predicted activities of a ketoreductase (dotA), oxidase (dotB), major facilitator superfamily transporter (dotC), and thioesterase (dotD). A D. pini dotA mutant was made by targeted gene replacement and shown to be severely impaired in dothistromin production, confirming that dotA is involved in dothistromin biosynthesis. Accumulation of versicolorin A (a precursor of aflatoxin) by the dotA mutant confirms that the dotA gene product is involved in an aflatoxin-like biosynthetic pathway. Since toxin genes have been found to be clustered in fungi in every case analyzed so far, it is speculated that the four dot genes may comprise part of a dothistromin biosynthetic gene cluster. A fifth gene, ddhA, is not a homolog of aflatoxin genes and could be at one end of the dothistromin cluster. These genes will allow comparative biochemical and genetic studies of the aflatoxin and dothistromin biosynthetic pathways and may also lead to new ways to control Dothistroma needle blight.
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